Strong Expression of Peroxisomal Malate Synthase Genes from n-Alkane-Utilizable Yeast Candida tropicalis in Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
Expression of the genes encoding several peroxisomal enzymes of the n-alkane-utilizable yeast Candida tropicalis has been examined in Saccharomyces cerevisiae to find out a novel and foreign promoter functional in S. cerevisiae. Among these genes, two malate synthase genes, as well as the isocitrate lyase gene, were strongly expressed in acetate-grown cells of S. cerevisiae. The amounts of the recombinant malate synthases expressed in S. cerevisiae were as much as 30% of the total amount of soluble proteins in the cells. These results showed that the upstream regions (1.1 kbp for the MS-1 gene and 0.8 kbp for the MS-2 gene) of the malate synthase genes [UPR-MSs] from C. tropicalis contained strong promoters functional in S. cerevisiae and that the construction of novel gene expression systems using UPR-MSs might be possible.
- 公益社団法人日本生物工学会の論文
- 1996-01-25
著者
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Atomi Haruyuki
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Atomi Haruyuki
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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MURAI Toshiyuki
Laboratory of Immunodynamics, Department of Microbiology and Immunology (C8), Osaka University Gradu
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Murai Toshiyuki
The Laboratory Of Molecular And Cellular Recognition Osaka University Graduate School Of Medicine
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Ueda Mitsuyoshi
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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TANAKA ATUSO
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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Tanaka Atuso
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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