Isolation and Characterization of Psychrotrophs from Subterranean Environments
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概要
- 論文の詳細を見る
Subterranean environments are potential sources for the isolation of novel microorganisms. Water and soil samples were collected at depths ranging from 10 to 1800 meters below the surface, and screening was carried out with aerobic rich and anaerobic minimal media. Two psychrotrophic and three chemoautotrophic strains were isolated. One of the psychrotrophic isolates, designated SN16A, grew at temperatures between -5 and 37℃ with optimal growth between 25 and 30℃. The other psychrotroph, designated KB700A, grew between -10 and 30℃. Little difference in growth rate could be observed between 20 and 30℃ ; however, this strain did not grow at 37℃. KB700A utilized CO_2 chemoautotrophically at 30℃, using hydrogen as an energy source. Both strains were characterized biochemically. The complete 16S rRNA sequence of KB700A was 98.7% homologous with that of Pseudomonas marginalis. However, the 16S rRNA of SN16A showed only 95.4% identity at maximum-with the corresponding gene of Arthrobacter globiformis-suggesting that this strain may belong to a novel genus. Both strains exhibited the ability to produce hydrolytic enzymes on plate assays. Our results suggest that subterranean environments are promising sources for the isolation of psychrotrophic microorganisms.
- 社団法人日本生物工学会の論文
- 1999-06-25
著者
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KIKUCHI HIROSHI
Department of Cardiology, Saiseikai Kumamoto Hospital
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IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
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EZAKI SATOSHI
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un
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Rashid Naeem
Dept. Synth. Chem. Biol. Chem. Grad. Sch. Eng. Kyoto Univ.:crest Imanaka Project
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ATOMI HARUYUKI
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto Un
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Atomi H
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Atomi Haruyuki
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Atomi Haruyuki
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Ezaki S
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Ezaki Satoshi
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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Ezaki Satoshi
Laboratory Of Molecular Gene Technics Graduate School Of Genetic Resources Technology Kyushu Univers
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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Atomi Haruyuki
Dept. Synth. Chem. Biol. Chem. Grad. Sch. Eng. Kyoto Univ.:crest Imanaka Project
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RASHID NAEEM
Core Research for Evolutional Science and Technology(CREST)
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Imanaka T
Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.
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Kikuchi H
Daiichi Pharmaceutical Co. Ltd. Tokyo Jpn
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