Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability

スポンサーリンク

概要

• 論文の詳細を見る

• Hyperthermophilic proteins possess many ion pairs on their surface. To reveal the role of the ion pairs, O^6-methylguanine-DNA methyltransferase from Thermococcus kodakaraensis KOD1 (Tk-MGMT) was studied as a model protein. The maximum free-energy changes of the protein in 0.1 and 0.5 M NaCl at pH 7.0 were 61.7 kJ mol^<-1> at 31.5℃ and 77.4 kJ mol^<-1> at 39.7℃, respectively. On the other hand, mid points of the thermal unfolding temperatures in 0.1 and 0.5 M NaCl at pH 7.0 were 94.8℃ and 90.1℃, respectively. The results suggest that the protein-surface ion pairs contribute to thermal stability (T_m), rather than thermodynamic stability (△G).

• 社団法人日本生物工学会の論文
• 2004-01-25

著者

• Takagi Masahiro

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi M

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• Takagi M

  Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn

• IMANAKA Tadayuki

  Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University

• Fujiwara S

  School Of Science And Engineering Department Of Life Science Kwansei-gakuin University

• Fujiwara Shinsuke

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• SHIRAKI KENTARO

  School of Materials Science, Japan Advanced Institute of Science and Technology

• NISHIKORI SHINGO

  School of Materials Science, Japan Advanced Institute of Science and Technology

• FUJIWARA SHINSUKE

  School of Science and Engineering, Departmeng of Life Science, Kwansei-Gakuin University

• Takagi M

  Nagasaki Univ. Nagasaki Jpn

• Imanaka Tadayuki

  Department Of Applied Biotechnology Faculty Of Engineering Osaka University

• Imanaka Tadayuki

  Department Of Biotechnology Faculty Of Eigineering Osaka University

• Imanaka Tadayuki

  Department Of Synthetic And Biological Chemistry Graduate School Of Engineering Kyoto University

• Imanaka T

  Department Of Biotechnology Faculty Of Engineering Osaka University

• Imanaka Tadayuki

  Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering.kyoto Univ

• Shiraki K

  Institute Of Applied Physics University Of Tsukuba

• Fujiwara Shinsuke

  School Of Science And Engineering Department Of Life Science Kwansei-gakuin University

• Takagi Mutsumi

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Takagi Masahiro

  School Of Material Science Japan Advanced Institute Of Science And Technology

• Imanaka T

  Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.

• Nishikori Shingo

  Division Of Molecular Cell Biology Institute Of Molecular Embryology And Genetics Kumamoto Universit

• Takagi Masahiro

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology (naist)

• Nishikori Shingo

  School of Materials Science, Japan Advanced Institute for Science and Technology

関連論文

• Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
• Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
• Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
• Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
• Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
• 1P245 酸化コレステロール含有膜小胞の熱応答ダイナミクス(生体膜・人工膜-ダイナミクス,第48回日本生物物理学会年会)
• Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
• Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Chondrocytes for Construction of Three-dimensional Cartilage Tissue
• Effect of Subcultivation of Human Bone Marrow Mesenchymal Stem on their Capacities for Chondrogenesis, Supporting Hematopoiesis, and Telomea Length
• Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
• Bacterial Alginate Lyase : Enzymology, Genetics and Application
• Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
• Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
• Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
• Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
• Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
• Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
• Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
• Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
• Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
• Development of hybrid artificial lung with gene transfected biological cells
• Gene Cloning and Sequence Analysis of Cobyric Acid Synthase and Cobalamin (5'-phosphate) Synthase from Hyperthermophilic Archaeon Pyrococcus sp. KOD1
• Biophysical Analysis of Heat-Induced Structural Maturation of Glutamate Dehydrogenase from a Hyperthermophilic Archaeon (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Mutational Effects on O^6-Methylguanine-DNA Methyltransferase from Hyperthermophile : Contribution of Ion-Pair Network to Protein Thermostability
• Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability
• Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1
• Biophysical Effect of Amino Acids on the Prevention of Protein Aggregation
• 超好熱菌由来タンパク質のさまざまな変性条件下での構造安定性
• Acceptor Specificity of 4-α-Glucanotransferase from Pyrococcus kodakaraensis KOD1, and Synthesis of Cycloamylose
• Characterization of FtsZ Homolog from Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1
• Characterization and Application to Hot Start PCR of Neutralizing Monoclonal Antibodies against KOD DNA Polymerase
• Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
• Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KOD1^1
• Phylogenetic Analysis and Effect of Heat on Conformational Change of Ferredoxin from Hyperthermophilic Archaeon Pyrococcus sp.KOD1
• Cloning and Expression of the 4-α-Glucanotransferase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
• Cloning and Expression of the α-Amylase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
• 453 Gene Cloning of Methylated DNA Protein Cysteine Methyltransferase (MGMT) from Pyrococcus sp. KOD1
• MALARIA ENDEMIC PATTERNS ON LOMBOK AND SUMBAWA ISLANDS, INDONESIA
• Analysis of relationship between anopheles subpictus larval densities and environmental parameters using remotote sensing (rs), a global positioning system (gps) and a geographic information system (gis)
• Vector Mosquitoes of Japanese Encephalitis (Diptera: Culicidae) in Northern Thailand: Seasonal Changes in Larval Community Structure
• SEASONAL AND SPATIAL DISTRIBUTION OF 3 MALARIA VECTORS AT THREE MOUNTAINOUS VILLAGES IN NORTH THAILAND
• SEASONAL DENSITY AND MALARIA VECTOR COMPETENCE OF ANOPHELES MINIMUS AND OTHER ANOPHELINES AT A SHALLOW VALLEY IN NORTH THAILAND
• Evaluation of effective period of a juvenile hormone mimic, pyriproxyfen, against Aedes albopictus : Preliminary experiments in the laboratory and the field.
• Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
• Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
• Movement of individually marked Aedes albopictus females in Nagasaki, Japan.
• Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
• Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
• Synthesis and Evaluation of Novel Peptide-Lipid
• Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
• Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
• Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
• Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
• 1116 Dye-metal affinity chromatography in mixed mode for purification of alcohol dehydrogenase
• 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
• Chelation of cadmium ions by phytochelatin synthas 　: role of the cystein-rich C-terminal
• Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
• Diamines Prevent Thermal Aggregation and Inactivation of Lysozyme(BIOCHEMICAL ENGINEERING)
• Equilibrium and Kinetic Stability of a Hyperthermophilic Protein, O^6-Methylguanine-DNA Methyltransferase under Various Extreme Conditions
• Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
• A Simple Method for Determination of Substrate Specificity of Alginate Lyases
• Purification and Characterization of α-Glucan Phosphorylase from Bacillus stearothermophilus
• Production and Some Properties of a Dextrin with a Narrow Size Distribution by the Cyclization Reaction of Branching Enzyme
• Expression of Recombinant Antibody against Cancer-Specific Carbohydrate
• COMPLETE GENOME ANALYSIS AND TARGETED GENE DISRUPTION IN THE HYPERTHERMOPHILIC ARCHAEON, THERMOCOCCUS KODAKARAENSIS(Life under Extreme Environments, International Symposium on Origins of Life and Astrobiology)
• Gene Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
• A Laboratory Experiment on the Larval Development of Culex tritaeniorhynchus (Diptera: Culicidae) Giles under Different Temperatures and Densities
• A Laboratory Experiment of The Predation by Possible Predators on Culex tritaeniorhynchus Larvae
• Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
• Combined Addition of Glutathione and Iron Chelators for Decrease of Intracellular Level of Reactive Oxygen Species and Death of Chinese Hamster Ovary Cells
• 1126 Effects of glutathione and iron chelators on level of intracellular reactive oxygren species
• Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
• Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin
• Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.
• Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
• 3TA3-06 酸化ストレスによる細胞サイズリポソームの動的構造変化(生体膜・人工膜-構造・物性,第47回日本生物物理学会年会)
• Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues
• Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
• Highly Thermostable Neutral Protease from Bacillus stearothemophilus
• Phytochelatins inhibit the metal-induced aggregation of α-crystallin(PLANT BIOTECHNOLOGY)
• Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
• Systematic Analysis of Aggregates from 38 Kinds of Non Disease-Related Proteins: Identifying the Intrinsic Propensity of Polypeptides to Form Amyloid Fibrils
• Diversity in Size and Alkaliphily of Thermostable α-Amylase-Pullulanases (AapT) Produced by Recombinant Escherichia coli, Bacillus subtilis and the Wild-Type Bacillus sp.
• Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
• Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
• Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
• Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
• Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
• Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
• Isolation and Characterization of Psychrotrophs from Subterranean Environments
• High-Level Production of Erythritol by Strain 618A-01 Isolated from Pollen
• Isolation of Bacterial Strans Colonizable in Mosquito Larval Guts as Novel Host Cells for Mosquito Control
• Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
• Dynamic Response of a Cholesterol-containing Model Membrane to Oxidative Stress
• Gentisate 1,2-Dioxygenase from Xanthobacter polyaromaticivorans 127W
• The Concept of the α-Amylase Family : Structural Similarity and Common Catalytic Mechanism
• Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
• Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
• Isolation of a novel alkaline-induced laccase from Flammulina velutipes and its application for hair coloring(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Isolation of a novel alkaline-induced laccase from Flammulina velutipes and its application for hair coloring

もっと見る 閉じる

スポンサーリンク