Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• We previously reported that a 3D coculture system for hematopoietic cells with spatial development of stromal cells in nonwoven fabrics was superior to a conventional 2D coculture system with respect to progenitor expansion and stem cell maintenance. The hematopoietic environments in 2D and 3D coculture systems were compared and it was found there were no differences in the amounts of soluble factors, e. g., mRNA levels of stem cell factor (SCF) and thrombopoietin (TPO), the secretion of SCF, and hematopoietic stimulating activity of culture supernatant. On the other hand, the total amount of insoluble factors such as extracellular proteins around the stromal cells and the mRNA level of laminin α5 were aparently higher in the 3D culture.

• 社団法人日本生物工学会の論文
• 2003-07-25

著者

• Soma T

  Department Of Medicine National Hospital Organization Osaka Minami Medical Center

• Soma Toshihiro

  大阪大学 医学系研究科

• Soma Toshihiro

  Medicine Iii Osaka University Medical School

• TAKAGI Mutsumi

  International Center for Biotechnology, Osaka University

• SOMA Toshihiro

  Osaka Minami National Hospital

• YOSHIDA Toshiomi

  International Center for Biotechnology, Osaka University

• SASAKI TAKAHIDE

  International Center for Biotechnology, Osaka University

• Yoshida Toshiomi

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Yoshida T

  International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• Takagi M

  International Center For Biotechnology Osaka University

• Takagi M

  Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn

• Takagi M

  Osaka Univ. Osaka Jpn

• Yoshida T

  Division Of Material And Biological Graduate School Japan Women's University

• Yoshida T

  Kinki Univ. Nara Jpn

• Yoshida Toyokazu

  Dept. Food Nutri. Fac. Agr. Kinki Univ.

• Shirakata Toshiaki

  Department Of Cancer Immunotherapy Osaka University Graduate School Of Medicine

• Takagi Mutsumi

  Internatioal Center For Biotechnology Osaka University

• Takagi Mutsumi

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Yoshida T

  Department Of Biomolecular Science Gifu University

• Yoshida Toshiomi

  Internatioal Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大 工

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