Physiologically Motivated Strategies for Control of the Fed-Batch Cultivation of Recombinant Escherichia coli for Phenylalanine Production
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概要
- 論文の詳細を見る
The efficiency of the fed-batch cultivation of recombinant Escherichia coli AT2471 for phenylalanine production is highly dependent on the distribution of the carbon flow between the main process products-biomass, phenylalanine, acetic acid and carbon dioxide. In order to enhance the process performance, the effects of several factors, namely glucose feeding, tyrosine feeding and oxygen supply, were investigated experimentally. As a result, a set of control strategies was developed, designed to tolerate phenylalanine synthesis at the expense of the remaining products. The DO was controlled to prevent acetic acid excretion due to oxygen limitation. The total amount of tyrosine fed was used to provide an optimal balance between biomass synthesis and that of phenylalanine. Special algorithms for control of the glucose feed rate were applied to eliminate the threat of acetic acid excretion due to overfeeding, and at the same time, to reduce excessive CO_2 evolution caused by unnecessarily severe glucose limitation. The joint application of these strategies resulted in greatly improved efficiency in the phenylalanine production process : the final phenylalanine concentration reached 46g/l, the yield was above 17%, and the productivity-0.85g/l・h. In combination, these data exceed the results reported by others, and are much higher than those obtained by us before the implementation of the proposed complex of techniques.
- 社団法人日本生物工学会の論文
- 1991-05-25
著者
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YOSHIDA Toshiomi
International Center for Biotechnology, Osaka University
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SEKI Tatsuji
International Center for Biotechnology, Osaka University
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Seki Tatsuji
International Center For Biotechnology Osaka University
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Seki Tatsuji
The International Center For Biotechnology Osaka University
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Yoshida Toshiomi
International Center For Biotechnology Osaka University
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吉田 豊和
岐阜大・工・生命工
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Konstantinov K
Osaka Univ. Osaka Jpn
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Konstantinov Konstantin
International Center Of Cooperative Research In Biotechnology Osaka University:(present Address)mile
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NISHIO NAOKI
International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka Univers
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Nishio Naoki
International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit
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Konstantinov Konstantin
International Center Of Cooperative Research In Biotechnology Eaculty Of Engineering Osaka Universit
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Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
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吉田 豊和
岐阜大 工
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