N-Linked Glycan Structures of a Mouse Monoclonal Antibody Produced from Tobacco BY2 Suspension-Cultured Cells(CELL AND TISSUE ENGINEERING)

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概要

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• cDNA encoding H- and L-chains from a mouse monoclonal antibody was introduced into tobacco BY2 cells, and the resulting sugar chain structures of plant-produced antibodies were analyzed by a combination of HPLC, exoglucosidase digestion and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The glycan structures determined were Man_<5-6>GlcNAc_2 (22.3%), GlcNAcMan_5GlcNAc_2 (3.1%), GlcNAcMan_3FucXylGlcNAc_2 (24.4%), GlcNAcMan_3XylGlcNAc_2 (17.8%), Man_3FucXylGlcNAc_2 (24.3%), and Man_3XylGlcNAc_2 (8.1%). The major glycan structures of the antibodies produced by transgenic suspension-cultured cells contain typical plant bisecting β(1,2)-xylose and α(1,3)-fucose residues, suggesting the posttranslational modification of a recombinant antibody in the late Golgi apparatus.

• 社団法人日本生物工学会の論文
• 2006-03-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• Omasa Takeshi

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Fujiyama Kazuhito

  The International Center for Biotechnology, Osaka University

• 大政 健史

  大阪大学大学院工学研究科

• Omasa Takeshi

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• MISAKI Ryo

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  The International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Misaki Ryo

  International Center For Biotechnology Osaka University

• Misaki Ryo

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  The International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Katsura Atsuya

  The International Center for Biotechnology, Osaka University

• Tanaka Tomoko

  The International Center for Biotechnology, Osaka University

• Furukawa Aiko

  The International Center for Biotechnology, Osaka University

• Furukawa Aiko

  The International Center For Biotechnology Osaka University

• Katsura Atsuya

  The International Center For Biotechnology Osaka University

• Tanaka Tomoko

  Dep. Of Life And Environment Engineering The Univ. Of Kitakyushu 1-1 Hibikino Wakamatsu-ku Kitakyush

• Fujiyama Kazuhito

  The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn

• Omasa Takeshi

  Department Of Biological Science And Technology Institute Of Technology And Science The University O

• Tanaka Tomoko

  The International Center For Biotechnology Osaka University

• Omasa Takeshi

  Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima

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