The Importance of Ionic Strength as a Parameter in Screening Peptide Ligands from a Phage Display Library
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概要
- 論文の詳細を見る
Peptide ligands which bound to a model monomeric protein, bovine pancreatic ribonuclease A, could be isolated from a constrained random hexapeptide phage library. Selection was successful in a low ionic strength buffer(10 mM sodium phosphate, pH 6.0), whereas it failed in TBS(50 mM Tris-Cl, 150 mM NaCl, pH 7.5). Two of the displayed amino acid sequences from among the clones isolated were AEGACEQLDYNC and AEGACLWHDQLC. Electrostatic interaction appeared to play an important role in the binding because these phages could not bind to RNase A at a high ionic strength. The results suggest that selection in low ionic strength buffers could make possible the isolation of peptide ligands against proteins of interest which do not originally interact with another peptide or protein.
- 社団法人日本生物工学会の論文
- 1998-04-25
著者
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Omasa Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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KATAKURA YOSHIO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Omasa Takeshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kurokawa Yuji
Department Of Environmental Technology And Urban Planning Graduate School Of Engineering Nagoya Inst
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SUGA KEN-ICHI
Department of Biotechnology, Faculty of Engineering, Osaka University
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Katakura Yoshio
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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LIM EUN
Department of Biotechnology, Graduate School of Engineering, Osaka University
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TSUJII SETSUO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Suga K
Osaka Univ. Osaka Jpn
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Suga K
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Suga K
Osaka Univ. Osaka
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Suga Ken-ichi
Department Of Biological Science And Technology Science University Of Tokyo
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Kurokawa Y
Division Of Cellular & Molecular Toxicology National Institute Of Health Sciences
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Lim Eun
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Tsujii Setsuo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kawasaki Yasushi
National Institute Of Health Sciences
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Omasa Takeshi
Department Of Biological Science And Technology Institute Of Technology And Science The University O
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Katakura Yoshio
Department Of Life Science And Bioengineering Faculty Of Chemistry Materials And Bioengineering Kans
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Omasa Takeshi
Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima
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