Hyaluronic acid production by recombinant Streptococcus thermophilus(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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Generally recognized as safe, Streptococcus thermophilus was transformed using a plasmid expressing endogenous hyaluronic acid (HA) synthase genes. A single expression of hyaluronic acid synthase (hasA), uridine diphosphate-glucose dehydrogenase gene (hasB), or pyrophosphorylase gene (glmU) and double expression of hasA and hasB were attempted. A streptococcus-Escherichia coli shuttle vector, pBE31, was successfully transfected in S. thermophilus. The single expression of hasA or hasB allowed S. thermophilus to produce about 0.5-1.0g/l HA. The strains coexpressing of hasA and hasB showed a markedly increased HA production (1.2g/l) which was six-fold increase compared with the wild-type strain. The maximum cell concentration and specific growth rate of each recombinant strain were lower than those of the wild-type strain; however, the specific production rate was more than 100-fold higher. Galactose concentration decreased in the coexpressing strain after depletion of lactose. The bacterial metabolism would be altered in order to achieve a higher production by changing the intracellular metabolism. The average molecular weight of HA (1.0×10^6Da) was not affected by the expression of hasA and hasB. HA produced from recombinant strain could be an alternative material for medical, cosmetic and food utilization instead of HA from conventional pathogenic streptococci.
著者
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Izawa Naoki
Yakult Central Institute for Microbiological Research
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Sone Toshiro
Yakult Central Institute for Microbiological Research
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Omasa Takeshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ohtake Hisao
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Serata Masaki
Yakult Central Institute for Microbiological Research
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Omasa Takeshi
Department Of Biological Science And Technology Institute Of Technology And Science The University O
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Omasa Takeshi
Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima
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