Streptococcus thermophilus produces exopolysaccharides including hyaluronic acid(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• Hyaluronic acid (HA) is an important material for medical, cosmetic and food applications. HA is obtained commercially from rooster combs and the fermentation of streptococci. However, the safety problems such as hyaluronidase or exotoxin contamination remain controversial. To reduce the risk of hyaluronidase or exotoxin contamination, we attempted to isolate strains of Streptococcus thermophilus with high productivity of useful exopolysaccharides (EPSs) including HA from traditional dairy food products. Forty-six S. thermophilus strains were isolated from dairy food products, and examined of their HA production using a HA binding protein method. According to the results, six S. thermophilus strains produced EPSs including HA. S. thermophilus YIT 2084 had a markedly high HA productivity (approximately 8mg/l). We focused on the high-molecular-mass fraction of EPS (2000kDa) from S. thermophilus YIT 2084. By using high-performance liquid chromatography, it was found that a high-molecular-mass fraction of EPS included N-acetylglucosamine (54.4%) and glucuronic acid (45.6%), which are components of HA. Furthermore, ^<13>C-nuclear magnetic resonance spectroscopy showed that the spectra of the high-molecular-mass fraction corresponded well to those of commercial HA. Here, we described for the first time that S. thermophilus, which is a generally recognized safe bacterium, produces HA. The novel HA-producing bacterium S thermophilus YIT 2084 has great potential for applications in the medical, cosmetic and food fields, although its culture conditions remain to be improved.

著者

• Izawa Naoki

  Yakult Central Institute for Microbiological Research

• Hanamizu Tomoko

  Yakult Central Institute for Microbiological Research

• Sone Toshiro

  Yakult Central Institute for Microbiological Research

• Chiba Katsuyoshi

  Yakult Central Institute for Microbiological Research

• Chiba Katsuyoshi

  ヤクルト中央研究所

• Izawa Naoki

  ヤクルト中央研究所

• Hanamizu Tomoko

  ヤクルト中央研究所

• Iizuka Ryoko

  Yakult Central Institute for Microbiological Research

• Mizukoshi Harumi

  Yakult Central Institute for Microbiological Research

• Kimura Kazumasa

  Yakult Central Institute for Microbiological Research

関連論文

• Effects of fermentation conditions and soybean peptide supplementation on hyaluronic acid production by Streptococcus thermophilus strain YIT 2084 in milk(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Streptococcus thermophilus produces exopolysaccharides including hyaluronic acid(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Effects of fermentation conditions and soybean peptide supplementation on hyaluronic acid production by Streptococcus thermophilus strain YIT 2084 in milk
• Effects of Prednisolone on the Cutaneous Reaction and Skin Barrier Function in Mice Treated with a Hapten
• Sialyl Oligosaccharides of Human Colostrum : Changes in Concentration during the First Three Days of Lactation
• Hyaluronic acid production by recombinant Streptococcus thermophilus(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク