Cloning and Characterization of Extracellular Metal Protease Gene of the Algicidal Marine Bacterium Pseudoalteromonas sp. Strain A28(Microbiology & Fermentation Technology)

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概要

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• The gene (empI) encoding an extracellular metal protease was isolated from a Pseudoalteromonas sp. strain A28 DNA library. The recombinant EmpI protein was expressed in E. coli and purified. Paper-disk assays showed that the purified protease had potent algicidal activity. A skim milk-polyacrylamide gel electrophoresis protease assay showed that the 38-kDa band of protease activity, which co-migrated with purified EmpI and was sensitive to 1,10-phenathroline, was detected in the extracellular supernatant of A28.

• 社団法人日本農芸化学会の論文
• 2002-06-23

著者

• Ohtake Hisao

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kato Junichi

  Department Of Cardiology Asahikawa Medical College

• KURODA Akio

  Department of Molecular Biotechnology, Hiroshima University

• Ohtake H

  Department Of Biotechnology Osaka University

• Ohtake Hisao

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Institute Of Applied Microbiology The University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• Kato J

  Department Of Molecular Biotechnology Hiroshima University

• Lee S‐o

  Hiroshima Univ. Hiroshima Jpn

• IKEDA TSUKASA

  Department of Fermentation Technology, Hiroshima University

• TAKIGUCHI NOBORU

  Department of Fermentation Technology, Hiroshima University

• LEE Sun-Og

  Department of Molecualr Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• NAKASHIMA Katsunori

  Department of Molecualr Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Lee Sun-og

  Department Of Molecualr Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Kuroda A

  Department Of Molecular Biotechnology Hiroshima University

• Kuroda Akio

  Department Of Molecular Biotechnology Hiroshima University

• Kato Junichi

  Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University

• Kato J

  Department Of Chemical Engineering Faculty Of Engineering Shizuoka University

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Ikeda T

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Takiguchi Noboru

  Department Of Molecular Biotechnology Hiroshima University

• Takiguchi Noboru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kato Junichi

  Department Of Agricultural Chemistry University Of Tokyo

• Ikeda T

  Osaka Medical Coll. Osaka

• Kuroda Akio

  Department Of Chemistry Faculty Of Science Nagoya University

• Nakashima Katsunori

  Department Of Molecualr Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

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