Production of α,ω-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4
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概要
- 論文の詳細を見る
Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed α,ω-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of α,ω-alkanediols from n-alkanes or 1-alkanols.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
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Kato Junichi
Department Of Cardiology Asahikawa Medical College
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ARISAWA Akira
Bioresource Laboratories, Mercian Corp.
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Fujii Tadashi
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Sumisa Futoshi
Bioresource Laboratories Mercian Corporation
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Kato Junichi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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Arisawa Akira
Bioresource Laboratories Mercian Corp.
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Arisawa Akira
Bioresource Laboratories Mercian Corporation
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NARIKAWA Tatsuya
Bioresource Laboratories, Mercian Corporation
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TAKEDA Koji
Bioresource Laboratories, Mercian Corporation
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Narikawa T
Bioresource Laboratories Mercian Corporation
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Narikawa Tatsuya
Department Of Chemistry College Of Science & Engineering Aoyama Gakuin University
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Takeda Koji
Bioresource Laboratories Mercian Corporation
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Fujii Tadashi
Bioresource Laboratories Mercian Co.
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