Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the Escherichia coli Expression System
スポンサーリンク
概要
- 論文の詳細を見る
Two hundred thirteen cytochrome P450 (P450) genes were collected from bacteria and expressed based on an Escherichia coli expression system to test their hydroxylation ability to testosterone. Twenty-four P450s stereoselectively monohydroxylated testosterone at the 2α-, 2β-, 6β-, 7β-, 11β-, 12β-, 15β-, 16α-, and 17-positions (17-hydroxylation yields 17-ketoproduct). The hydroxylation site usage of the P450s is not the same as that of human P450s, while the 2α-, 2β-, 6β-, 11β-, 15β-, 16α-, and 17-hydroxylation are reactions common to both human and bacterial P450s. Most of the testosterone hydroxylation catalyzed by bacterial P450s is on the β face.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
-
ARISAWA Akira
Bioresource Laboratories, Mercian Corp.
-
Ishikawa Jun
National Research Laboratory Of Metrology
-
KABUMOTO Hiroki
Bioresource Laboratories, Mercian Corporation
-
Fujii Yoshikazu
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
-
Doi Satoru
Bioresource Laboratories Mercian Corp.
-
Arisawa Akira
Bioresource Laboratories Mercian Corp.
-
Agematu Hitosi
Bioresource Laboratories Mercian Corp.
-
MATSUMOTO Naoki
Bioresource Laboratories, Mercian Corp.
-
MACHIDA Kazuhiro
Bioresource Laboratories, Mercian Corp.
-
Kabumoto Hiroki
Bioresource Laboratories Mercian Corp.
-
Machida Kazuhiro
Bioresource Laboratories Mercian Corp.
-
Matsumoto Naoki
Bioresource Laboratories Mercian Corp.
-
Ishikawa Jun
National Institute Of Infectious Diseases
-
Agematu Hitosi
Dep. Of Applied Chemistry Akita National Coll. Of Technol.
関連論文
- Generation of New Benanomicin Analogues by Biotransformation Using Escherchia coli Expressing Actinomycete Cytochrome P450
- Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor
- Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes
- Optical Frequency Measurement Using Chirped-Mirror-Dispersion-Controlled Mode-Locked Ti:Al_2O_3 Laser
- The Novel Enzymatic 3"-N-Acetylation of Arbekacin by an Aminoglycoside 3-N-Acetyltransferase of Streptomyces Origin and the Resulting Activity
- Enzymatic 2'-N-Actylation of Arbekacin and Antibiotic Activity of Its Product
- Mechanism of Multiple Aminoglycoside Resistance of Kasugamycin-producing Streptomyces kasugaensis MB273: Involvement of Two Types of Acetyltransferases in Resistance to Astromicin Group Antibiotics
- PCR Inhibition Assay for DNA-targeted Antibiotics
- Increase in the Rate of L-Pipecolic Acid Production Using lat-Expressing Escherichia coli by lysP and yeiE Amplification(Microbiology & Fermentation Technology)
- Production of α,ω-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4
- Accurate Wavelength Measurement of the Intercombination Line of Calcium
- Velocity Selective Absorption by a Long Lifetime Transition in a Ca Atomic Beam
- Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the Escherichia coli Expression System
- Crystal structure of cytochrome P450 MoxA from Nonomuraea recticatena (CYP105)
- Hydroxylation of Oleanolic Acid to Queretaroic Acid by Cytochrome P450 from Nonomuraea recticatena
- Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete chrysosporium
- Organization of the Biosynthetic Gene Cluster for the Polyketide Antitumor Macrolide, Pladienolide, in Streptomyces platensis Mer-11107
- Production of Human Metabolites of Cyclosporin A, AM1, AM4N and AM9, by Microbial Conversion(Microbial Physiology and Biotechnology)
- Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes
- Increase in Pladienolide D Production Rate Using a Streptomyces Strain Overexpressing a Cytochrome P450 Gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Directed Evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for Enhanced Activity
- Efficient Biotransformations Using Escherichia coli with tolC acrAB Mutations Expressing Cytochrome P450 Genes
- A Compact I2-Stabilized 532 nm Nd:YAG Laser
- One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Biotransformation of L-Lysine to L-Pipecolic Acid Catalyzed by L-Lysine 6-Aminotransferase and Pyrroline-5-carboxylate Reductase(Microbiology & Fermentation Technology)
- High-Sensitive Spectroscopy of Molecular Iodine by Means of Power-Stabilized Light from a Diode Laser Spectrometer