Hydroxylation of Oleanolic Acid to Queretaroic Acid by Cytochrome P450 from Nonomuraea recticatena
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概要
- 論文の詳細を見る
A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
- 社団法人 日本農芸化学会の論文
- 2007-09-23
著者
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Fujii Yoshikazu
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Fujii Tadashi
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Arisawa Akira
Bioresource Laboratories Mercian Corp.
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Agematu Hitosi
Bioresource Laboratories Mercian Corp.
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MATSUMOTO Naoki
Bioresource Laboratories, Mercian Corp.
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Hirosue Shinji
Bioresource Laboratories Mercian Corp.
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Fujii Tadashi
Bioresource Laboratories Mercian Co.
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Matsumoto Naoki
Bioresource Laboratories Mercian Corp.
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Agematu Hitosi
Dep. Of Applied Chemistry Akita National Coll. Of Technol.
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Hirosue Shinji
Bioresource Laboratories, Mercian Corp.
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