The Fibrinolytic Activity of a Novel Protease Derived from a Tempeh Producing Fungus, Fusarium sp. BLB
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概要
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Tempeh is a traditional Indonesian soybean-fermented food produced by filamentous fungi, Rhizopus sp. and Fusarium sp. We isolated and sequenced the genomic gene and a cDNA clone encoding a novel protease (FP) from Fusarium sp. BLB. The genomic gene was 856 bp in length and contained two introns. An isolated cDNA clone encoded a protein of 250 amino acids. The predicted amino acid sequence of FP showed highest homology, of 76%, with that of trypsin from Fusarium oxysporum. The hydrolysis activity of FP toward synthetic peptide was higher than that of any other protease tested, including Nattokinases. Furthermore, the thrombolytic activity of FP was about 2.1-fold higher than that of Nattokinase when the concentration of plasminogen was 24 units/ml. These results suggest that FP is superior to Nattokinases in dissolving fibrin when absorbed into the blood.
- 社団法人 日本農芸化学会の論文
著者
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Fujii Tadashi
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Johdo Osamu
Bioresource Laboratories Mercian Co.
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Nakamura Takumi
Sodx Co. 206-1 Kagata Kawachinagano
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SUGIMOTO Satoshi
Bioresource Laboratories, Mercian Co.
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MORIMIYA Tatsuo
Bioresource Laboratories, Mercian Co.
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Morimiya Tatsuo
Bioresource Laboratories Mercian Co.
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Sugimoto Satoshi
Bioresource Laboratories Mercian Co.
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Fujii Tadashi
Bioresource Laboratories Mercian Co.
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FUJII Tadashi
Bioresource Laboratories, Mercian Co.
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JOHDO Osamu
Bioresource Laboratories, Mercian Co.
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NAKAMURA Takumi
Sodx Co.
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