Increase in the Rate of L-Pipecolic Acid Production Using lat-Expressing Escherichia coli by lysP and yeiE Amplification(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Biotransformation of L-lysine (L-Lys) to L-pipecolic acid (L-PA) using lat-expressing Escherichia coll has been reported (Fujii et al., Biosci. Biotechnol. Biochem., 66, 622-627 (2002)). The rate-limiting step of this biotransformation seemes to be the transport of L-Lys into cells. To improve the L-PA production rate, we attempted to increase the rate of L-Lys uptake. E. coli BL21 carrying a plasmid with lat and lysP (pRH125) caused a 5-fold increase in the rate of L-PA production above the level of cells carrying a plasmid with lat (pRH124). Moreover, E. coli BL21 carrying a plasmid with lat, lysP, and yeiE (pRH127) caused a 6.4-fold increase in the rate of L-PA production above the level of cells carrying pRH124. Our results from RT-PCR experiments and the sequence similarity of YeiE to LysR transcriptional regulators suggest the possibility that yeiE expression induces lysP expression. The amplification of lysP, or rather both lysP and yeiE, increases the rate of L-PA production using lat-expressing E. coli.
- 社団法人日本農芸化学会の論文
- 2002-09-23
著者
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Fujii Tadashi
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Agematsu Hitoshi
Bioresource Laboratories Mercian Corp.
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Agematu Hitosi
Bioresource Laboratories Mercian Corp.
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ARITOKU Yasuhide
Bioresource Laboratories, Mercian Corp.
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TSUNEKAWA Hiroshi
Bioresource Laboratories, Mercian Corp.
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Aritoku Yasuhide
Bioresource Laboratories Mercian Corp.
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Tsunekawa Hiroshi
Bioresource Laboratories Mercian Corp.
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Fujii Tadashi
Bioresource Laboratories Mercian Co.
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