Biotransformation of Various Alkanes Using the Escherichia coli Expressing an Alkane Hydroxylase System from Gordonia sp. TF6
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概要
- 論文の詳細を見る
Biotransformation using alkane-oxidizing bacteria or their alkane hydroxylase (AH) systems have been little studied at the molecular level. We have cloned and sequenced genes from Gordonia sp. TF6 encoding an AH system, alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin), and rubB (rubredoxin reductase). When expressed in Escherichia coli, these genes allowed the construction of biotransformation systems for various alkanes. Normal alkanes with 5 to 13 carbons were good substrates for this biotransformation, and oxidized to their corresponding 1-alkanols. Surprisingly, cycloalkanes with 5 to 8 carbons were oxidized to their corresponding cycloalkanols as well. This is the first study to achieve biotransformation of alkanes using the E. coli expressing the minimum component genes of the AH system. Our biotransformation system has facilitated assays and analysis leading to improvement of AH systems, and has indicated a cycloalkane oxidation pathway in microorganisms for the first time.
- 社団法人 日本農芸化学会の論文
- 2004-10-23
著者
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Kato Junichi
Department Of Cardiology Asahikawa Medical College
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Fujii Tadashi
Bioresource Laboratories Mercian Corp. 1808 Nakaizumi Iwata Shizuoka 438-0078 Jpn
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Kato Junichi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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NARIKAWA Tatsuya
Bioresource Laboratories, Mercian Corporation
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TAKEDA Koji
Bioresource Laboratories, Mercian Corporation
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Narikawa T
Bioresource Laboratories Mercian Corporation
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Narikawa Tatsuya
Department Of Chemistry College Of Science & Engineering Aoyama Gakuin University
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Takeda Koji
Bioresource Laboratories Mercian Corporation
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Fujii Tadashi
Bioresource Laboratories Mercian Co.
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