Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp. SBH81 and Its Tolerance Mechanism
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概要
- 論文の詳細を見る
A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
- 日本微生物生態学会・日本土壌微生物学会の論文
- 2012-03-01
著者
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Vangnai Alisa
Department Of Biochemistry Faculty Of Science Chulalongkorn University:national Center Of Excellence
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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Tajima Takahisa
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kongpol Ajiraporn
Graduate Program In Biotechnology Faculty Of Science Chulalongkorn University
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Tajima Takahisa
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Vangnai Alisa
Department Of Biochemistry Faculty Of Science Chulalongkorn University
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Kato Junichi
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Alisa S.Vangnai
Department of Biochemistry, Faculty of Science, Chulalongkorn University
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