Transcriptional Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11

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概要

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• The nitrifying bacterium Nitrosomonas sp. strain ENI-11 has three copies of the gene encoding hydroxylamine oxidoreductase (hao1, hao2, and hao3) on its genome. Broad-host-range reporter plasmids containing transcriptional fusion genes between hao copies and lacZ were constructed to analyze the expression of each hydroxylamine oxidoreductase gene (hao) copy individually and quantitatively. β-Galactosidase assays of ENI-11 harboring reporter plasmids revealed that all hao copies were transcribed in the wild-type strain. Promoter analysis of hao copies revealed that transcription of hao3 was highest among the hao copies. Expression levels of hao1 and hao2 were 40% and 62% of that of hao3 respectively. Transcription of hao1 was negatively regulated, whereas a portion of hao3 transcription was read through transcription from the rpsT promoter. When energy-depleted cells were incubated in the growth medium, only hao3 expression increased. This result suggests that it is hao3 that is responsible for recovery from energy-depleted conditions in Nitrosomonas sp. strain ENI-11.

• 社団法人 日本農芸化学会の論文
• 2006-08-23

著者

• Ohtake Hisao

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kato Junichi

  Department Of Cardiology Asahikawa Medical College

• KURODA Akio

  Department of Molecular Biotechnology, Hiroshima University

• Ohtake H

  Department Of Biotechnology Osaka University

• Ohtake Hisao

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Institute Of Applied Microbiology The University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• Hirota Ryuichi

  Department of Molecular Biotechnology, Hiroshima University

• Kato J

  Department Of Molecular Biotechnology Hiroshima University

• IKEDA TSUKASA

  Department of Fermentation Technology, Hiroshima University

• TAKIGUCHI NOBORU

  Department of Fermentation Technology, Hiroshima University

• Okumura H

  Electrotechnical Lab. Ibaraki Jpn

• Ohtake Hisao

  大阪大学 工学研究科生命先端工学専攻

• Kuroda A

  Department Of Molecular Biotechnology Hiroshima University

• Kuroda Akio

  Department Of Molecular Biotechnology Hiroshima University

• Kato Junichi

  Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University

• Kato J

  Department Of Chemical Engineering Faculty Of Engineering Shizuoka University

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Ikeda T

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Takiguchi Noboru

  Department Of Molecular Biotechnology Hiroshima University

• Takiguchi Noboru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kato Junichi

  Department Of Agricultural Chemistry University Of Tokyo

• Hirota Ryuichi

  Department Of Molecular Biotechnology Hiroshima University

• Ikeda T

  Osaka Medical Coll. Osaka

• Kuroda Akio

  Department Of Chemistry Faculty Of Science Nagoya University

• Hirota Ryuichi

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University

• Okumura Hajime

  Department of Biotechnology, Graduate School of Engineering, Osaka University

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