Isolation and Characterization of the Enterobacter cloacae cheR Mutant Defective in Phosphate Taxis
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概要
- 論文の詳細を見る
A chemotaxis-defective mutant of Enterobacter cloacae IFO3320,designated EC1,was isolated after N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. Computer-assisted capillary assays showed that EC1 failed to show chemotactic responses to peptone and inorganic phosphate (P_i). Cloning and sequence analysis showed that EC1 is a cheR mutant, suggesting that P_i taxis by E. cloacae is dependent on a methyl-accepting chemotaxis protein(s)(MCP). EC1 was further mutagenized with NTG to construct cheR pstS and cheR pstA double mutants. A recombinant plasmid pECT01.2,which contained the E. cloacae cheR gene, restored the ability of these double mutants to show chemotaxis toward peptone but not P_i. These results suggest that the phosphate-specific transport (Pst) system, together with a MCP(s), is required for detecting P_i in E. cloacae.
- 社団法人日本農芸化学会の論文
- 2001-02-23
著者
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Ohtake Hisao
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kato Junichi
Department Of Cardiology Asahikawa Medical College
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KURODA Akio
Department of Molecular Biotechnology, Hiroshima University
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Ohtake H
Department Of Biotechnology Osaka University
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Ohtake Hisao
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ohtake H
Institute Of Applied Microbiology University Of Tokyo
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Ohtake Hisao
Institute Of Applied Microbiology The University Of Tokyo
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Ohtake Hisao
Department Of Fermentation Technology Hiroshima University
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Yang Li
中華人民共和国
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Yang Li
Department Of Obstetrics And Gynecology Miyazaki Medical College
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IKEDA TSUKASA
Department of Fermentation Technology, Hiroshima University
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TAKIGUCHI NOBORU
Department of Fermentation Technology, Hiroshima University
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NAGATA Chinatsu
Department of Fermentation Technology, Hiroshima University
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Yang Li
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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Kuroda A
Department Of Molecular Biotechnology Hiroshima University
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Kuroda Akio
Department Of Molecular Biotechnology Hiroshima University
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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Kato J
Department Of Chemical Engineering Faculty Of Engineering Shizuoka University
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Ikeda Tsukasa
Department Of Applied Chemistry Utsunomiya University
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Ikeda T
Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.
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Nagata Chinatsu
Department Of Fermentation Technology Hiroshima University
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Takiguchi Noboru
Department Of Molecular Biotechnology Hiroshima University
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Takiguchi Noboru
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Yang Li
Department Of Biotechnology Graduate School Of Agicultural And Life Sciences The University Of Tokyo
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Ikeda T
Osaka Medical Coll. Osaka
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Kuroda Akio
Department Of Chemistry Faculty Of Science Nagoya University
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Yang Li
Department of Bioengineering, Chongqing University
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