Spheroplast Fusion of Acetobacter aceti and Its Application to the Breeding of Strains for Vinegar Production(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• Spheroplasts of auxotrophic mutants derived from Acetobacter aceti subsp. aceti No. 1023 were efficiently prepared by treatment with lysozyme, using sucrose as an osmotic stabilizer, and regenerated on an agar plate containing sorbitol and gelatin. In addition, spheroplast fusion between the several auxotrophic mutants was achieved in the presence of polyethylene glycol and CaCl_2. The frequency of fusion was found to be about 5x10^<-5>. Spheroplast fusion between A. aceti subsp. aceti No. 2 with the ability to grow at high temperature and A. aceti subsp. xylinum NBI1002 with high resistance to acetic acid was also achieved by the same method, with a frequency of 6.0x10^<-6>. The fusants showed various degrees of resistance to acetic acid and ability to grow at high temperature. One of the fusants, named No. 116, could produce acetic acid from ethanol continuously under conditions under which both parent strains were unable to grow. This suggests that spheroplast fusion is applicable to the breeding of strains for vinegar production.

• 社団法人日本農芸化学会の論文
• 1989-09-23

著者

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Institute Of Applied Microbiology The University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• FUKAYA MASAHIRO

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• OKUMURA HAJIME

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• KAWAMURA YOSHIYA

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• Fukuwaka Masa-aki

  Hokkaido National Fisheries Research Institute

• Toda K

  Univ. Tokyo Tokyo Jpn

• Toda K

  Department Of Applied Chemistry And Biotechnology Niigata Institute Of Technology

• Toda K

  Faculty Of Engineering Niigata Institute Of Technology

• BEPPU Teruhiko

  Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok

• Tayama Kenji

  Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.

• Takemura Hiroshi

  Bio-polymer Research Co. Ltd.:(present Address)nakano Central Institute Nakano Vinegar Co. Ltd.

• Beppu T

  Department Of Applied Biological Science Nihon University

• Toda Kiyoshi

  Faculty Of Engineering Niigata Institute Of Technology

• Kawamura Y

  National Food Research Inst. Tsukuba Jpn

• TAGAMI Haruko

  Nakano Central Research Institute, Nakano Vinegar Co., Ltd.

• Kawamura Y

  Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.

• Fukaya M

  Central Research Institute Mitsukan Group Corporation

• Tagami Haruko

  Nakano Central Research Institute Nakano Vinegar Co. Ltd.

• Beppu Teruhiko

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• FUKAYA Masahiro

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

• OKUMURA Hajime

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

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