Role of Intracellular Esterases in the Production of Esters by Acetobacter pasteurianus

スポンサーリンク

概要

• 論文の詳細を見る

• Esters are the major flavor compounds produced by Acetobacter sp. during vinegar production. The two genes encoding the esterases in the bacteria were disrupted, and the effects of the disruptions studied. When cultured in the presence of ethanol, the est1 gene-disrupted mutant (DE1K) did not produce any ethyl acetate or isoamyl acetate. However, the disruption of est2 did not affect the ester production. Ethyl acetate production by N-23 (pME122P) and DE1K (pME122P), which contain est1, was 1.7-fold higher than that by the wild type, N-23. On analyzing the relationship between ethyl acetate production and the extracellular ethanol and acetic acid concentrations, we found that the highest amount of ethyl acetate was produced when the molar ratio of ethanol and acetic acid was 1:1. These results indicate that the ester production by Acetobacter sp. is mostly catalyzed by the intracellular esterase, esterase-1, with ethanol and acetic acid used as the substrates.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• Koizumi Yukimichi

  Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture

• NAKANO Takayuki

  Department of Electrical and Electronic Engineering, Niigata University

• Toda K

  Faculty Of Engineering Niigata Institute Of Technology

• Kawamura Yukio

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Kawamura Y

  Akita Res. Inst. Food And Brewing (arif) Akita

• Kawamura Y

  National Food Res. Inst. Ibaraki Jpn

• UDAKA SHIGEZO

  Department of Fermentation Sciences, Tokyo University of Agriculture

• Kashima Y

  National Inst. Of Advanced Industrial Sci. And Technol. (aist Kansai) Osaka Jpn

• Koizumi Y

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• KASHIMA Yasuhiro

  Department of Fermentation Science, Tokyo University of Agriculture

• YANAGIDA Fujiharu

  Department of Fermentation Science, Tokyo University of Agriculture

• TAYAMA Kenji

  Central Research Institute, Nakano Vinegar Co., Ltd.

• Tayama K

  Mitsukan Group Corp. Aichi Jpn

• Tayama Kenji

  Central Research Institute Mitsukan Group Corporation

• Tayama Kenji

  Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.

• Kashima Yasuhiro

  Department Of Chemistry Oklahoma State University

• Yanagida Fujiharu

  Department Of Fermentation Science Faculty Of Applied Bioscience Tokyo University Of Agriculture

• Udaka S

  Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Udaka Shigezo

  Department Of Brewing And Fermentation Tokyo University Of Agriculture

• IIJIMA MASUMI

  Department of Fermentation Science, Tokyo University of Agriculture

• Nakano Takayuki

  Department Of Biosciences Teikyo University

• Koizumi Yukimichi

  Department Of Fermentation Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Koizumi Y

  Kyoto Univ. Kyoto Jpn

• Iijima Masumi

  Department Of Fermentation Science Tokyo University Of Agriculture

• Udaka Shigezo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Nakano Takayuki

  Department Of Fermentation Science Tokyo University Of Agriculture

• YANAGIDA Fujiharu

  Department of Brewing and Fermentation, Tokyo University of Agriculture

関連論文

• Cloning and Characterization of clpB in Acetobacter pasteurianus NBRC3283(BREWING AND FOOD TECHNOLOGY)
• Cloning and characterization of grpE in Acetobacter pasteurianus NBRC3283(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Enhancement of Acetic Acid Production in a High Cell-Density Culture of Acetobacter aceti
• Effects of Dissolved Oxygen and Acetic Acid Concentrations on Acetic Acid Production in Continuous Culture of Acetobacter aceti
• Emission Light and Multiple Surface Plasmon Excitations at Prism/Ag/Merocyanine Langmuir-Blodgett Films
• Effects of Nutrient-Poor and Anaerobic Conditions on Maintenance of R100-1 and RSF2124 Plasmids in Model Populations of Escherichia coli
• Stability of Plasmid-Bearing Microorganisms in Batch and Continuous Cultures
• Isolation of a Protease-Deficient Mutant of Bacillus brevis and Efficient Secretion of a Fungal Protein Disulfied Isomerase by the Mutant
• Genetic Transformation of Bacillus brevis with Plasmid DNA by Electroporation(Microbiology & Fermentation Industry)
• Potential Use of Bacillus brevis HPD31 for the Production of Foreign Proteins(Microbiology & Fermentation Industry)
• Screening and Characterization of Protein-hyperproducing Bacteria without Detectable Exoprotease Activity(Microbiology & Fermentation Industry)
• Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124
• Factors Affecting Conjugative Transfer Rates of Plasmids in Batch and Continuous Cultures
• Novel Benzacridine Derivative in the Green Pigment from Methyl Caffeate and Butylamine
• Development of Free Radical Products during the Greening Reaction of Caffeic Acid Esters (or Chlorogenic Acid) and a Primary Amino Compound(Food & Nutrition Science)
• Structure of Green Pigment Formed by the Reaction of Caffeic Acid Esters (or Chlorogenic acid) with a Primary Amino Compound(Food & Nutrition Science)
• Lateral Polarity Control in GaN Based on Selective Growth Procedure Using Carbon Mask Layers
• A Gene Encoding Phosphatidylethanolamine N-Methyltransferase from Acetobacter aceti and Some Properties of its Disruptant(Microbiology & Fermentation Technology)
• MUP1,High Affinity Methionine Permease, is Involved in Cysteine Uptake by Saccharomyces cerevisiae
• The est1 Regulation Depends on the Oxygen Concentration in Acetobacter pasteurianus
• A Permease Exhibiting a Dual Role for Lysine and Biotin Uptake in Saccharomyces cerevisiae
• High Effeciency Transformation of Bacillus brevis by Electroporation
• Protein Disulfide Isomerase Activity of Some Plant Seeds
• Adxanthromycins A and B, New Inhibitors of ICAM-1/LFA-1 Mediated Cell Adhesion Molecule from Streptomyces sp. NA-148 : II. Physico-chemical Properties and Structure Elucidation
• Adxanthromycin: A New Inhibitor of ICAM-1/LFA-1 Mediated Cell Adhesion from Streptomyces sp. NA-148
• Molecular Cloning of a Fungal cDNA Encoding Protein Disulfide Isomerase
• Purification of Protein Disulfide Isomerase from a Thermophilic Fungus
• Enhanced Oxygen Absorption in Shallow-Flow Surface Culture
• Bacterial Reduction of Toxic Hexavalent Chromium Using a Fed-Batch Culture of Enterobacter cloacae Strain HOl
• Accumulation of S-2-Hydroxyethyl Derivatives of L-Cysteine and L-Homocysteine by a Methionine Auxotroph of Escherichia coli with the Addition of 2-Mercaptoethanol to the Culture(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Accumulation of _L-Cystathionine by an Escherichia coli Mutant Deficient in Cystathionine Beta-Lyase
• Substantially Complete Removal of the 34 kDa Allergenic Soybean Protein, Gly m Bd 30 K, from Soy Milk of a Mutant Lacking the α- and α'-Subunits of Conglycinin
• Molecular Cloning of Acid-Stable Glucose Isomerase Gene from Streptomyces olivaceoviridis E-86 by a Simple Two-Step PCR Method, and Its Expression in Escherichia coli
• Identification of Functionally Important Cysteine Residues of the Haman Renin-Binding Protein as the Enzyme N-Acetyl-D-Glucosamine 2-Epimerase 1
• Renin Inhibits N-Acetyl-D-Glucosamine 2-Epimerase (Renin-Binding Protein)^1
• Construction of a Chimeric Shuttle Plasmid via a Heterodimer System : Secretion of an scFv Protein from Bacillus brevis Cells Capable of Inhibiting Hemagglutination
• Random Point Mutation Analysis of the Signal Peptide Cleavage Area of Bacillus stearothermophilus α-Amylase(Microbiology & Fermentation Industry)
• Accumulation and Degradation of Thiamin-binding Protein and Level of Thiamin in Wheat Seeds during Seed Maturation and Germination
• Effects of anesthesia upon ^F-FDG uptake in rhesus monkey brains
• Secretory Production of Erythropoietin and the Extracellular Domain of the Erythropoietin Receptor by Bacillus brevis : Affinity Purification and Characterization
• Purification and Characterizationof Protein Disulfide Isomerase from Soybeam
• Gene Expression in Bacillus brevis under Control of the Lac Repressor-Operator System from Escherichia coli
• Production of Human Epidermal Growth Factor by Bacillus brevis Increased with Use of a Stable Plasmid from B. brevis 481
• 565 Charcteristics of Batch Culture of a Recombinant ___- ___- Producing a Foreign Esterase
• Analysis by Deletion and Site-directed Mutagenesis of Promoters of the Cell Wall Protein Gene Operon in Bacillus brevis 47(Microbiology & Fermentation Industry)
• β-Lactotensin, a Neurotensin NT_2 Agonist, Stimulates Bile Acid Secretion
• Physiological effects and mechanism of Arg-Ile-Tyr, a multifunctional peptide derived from rapeseed protein
• Effects of dietary polyamines on the promotion of mammary tumor in rats.
• Effects of Dietary Polyamines on the Promotion of Mammary Tumor in Rats
• Cloning and Characterization of the dnaKJ Operon in Acetobacter aceti (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Cloning and Characterization of groESL Operon in Acetobacter aceti
• Exploration of Thermophilic Esterases/Lipases for Asymmetric Desymmetrization of Norbornane Derivatives
• Role of Intracellular Esterases in the Production of Esters by Acetobacter pasteurianus
• Cloning and Characterization of Ethanol-Regulated Esterase Genes in Acetobacter pasteurianus
• Angiotensin I Converting Enzyme Inhibitory Activities of Various Fermented Foods
• Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers(Food & Nutrition Science)
• Purification and Characterization of Intracellular Esterases Related to Ethylacetate Formation in Acetobacter pasteurianus
• Polyamine Content of Ordinary Foodstuffs and Various Fermented Foods
• Antihypertensive Effects of Acetic Acid and Vinegar on Spontaneously Hypertensive Rats(Food & Nutrition Science)
• Efficient Production of Bacillus stearothermophilus α-Amylase in Bacillus brevis by Altering Its Signal Peptide
• Theoretical Correlation between Viscosities at Dynamic and Steady Flow States in Aureobasidium pullulans Culture Fluids(BIOCHEMICAL ENGINEERING)
• A Modified Model for Non-Newtonian Viscosity behavior of Aureobasidium pullulans Culture Fluid
• Characteristic Behavior of Viscosity and Viscoelasticity of Aureobasidium pullulans Culture Fluid
• Fluid Viscosity of Aureobasidium pullulans Cultures Obtained at Two Different Initial pH Values
• Analysis of Flow Behavior of Non-Newtonian Fluids Based on a Concept of Traveling Force
• Cell Concentration Dependence of Dynamic Viscoelasticity of Escherichia coli Culture Suspensions
• Cellulose Production by Acetic Acid-Resistant Acetobacter xylinum
• Transformation of Acetobacter polyoxogenes with Plasmid DNA by Electroporation
• The aarC Gene Responsible for Acetic Acid Assimilation Confers Acetic Acid Resistance on Acetobacter aceti
• Spheroplast Fusion of Acetobacter aceti and Its Application to the Breeding of Strains for Vinegar Production(Microbiology & Fermentation Industry)
• A Kinetic Study on Total Degradation of 4-Chlorobiphenyl by a Two-Step Culture of Arthrobacter and Pseudomonas Strains
• Characterization of Indium Segregation in Metalorganic Vapor Phase Epitaxy-Grown InGaP by Schottky Barrier Height Measurement
• Production of Thermostable Clostridium thermohydrosulfuricum Xylose Isomerase in Bacillus brevis(Microbiology & Fermentation Industry)
• Xylose (Glucose) Isomerase Gene from the Thermophile Clostridium thermohydrosulfuricum; Cloning, Sequencing, and Expression in Escherichia coli(Microbiology & Fermentation Industry)
• Efficient Production of N-terminally Truncated Biologically Active Human Interleukin-6 by Bacillus brevis
• Efficient Production of Thermostable Clostridium thermosulfurogenes β-Amylase by Bacillus brevis
• 107 Effective Extracellular Production of Bacillus stearothermophilus Esterase by pH-stat modal Fed-batch Culture of Recombinant Bacillus brevis
• Hydrogen Peroxide Resistance of Acetobacter pasteurianus NBRC3283 and Its Relationship to Acetic Acid Fermentation
• Seebeck Effects and Electronic Thermal Conductivity of IV-VI Materials
• Purification and Properties of Divalent Cation-Dependent Raw-Starch-Digesting α-Amylase from Bacillus sp. B1018
• Adxanthromycins A and B, New Inhibitors of ICAM-1/LFA-1 Mediated Cell Adhesion Molecule from Streptomyces sp. NA-148 : I. Taxonomy, Production, Isolation and Biological Activities
• Characterization of rpoH in Acetobacter pasteurianus NBRC3283(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Structure of an Acidic Polysaccharide Elaborated by Acetobacter sp. NBI 1005(Biological Chemistry)
• Study of the internal structure of the Kuranosuke snow patch in central Japan using ground penetrating radar survey
• Secretion of Human Interleukin-2 in Biologically Active Form by Bacillus brevis Directly into Cultute Medium
• BbrPI, an Extracellular Proteinase Inhibitor of Bacillus brevis, Protects Cells from the Attack of Exogenous Proteinase
• Isolation of Peptides from an Enzymatic Hydrolysate of Food Proteins and Characterization of Their Taste Properties
• Optimal Culture Conditions for Production of the Macromolecular Peptide Antibiotics AN-1,AN-3 and AN-7
• Sweetness of Lysozymes
• Reproducibility of Arterial Blood Gases Determined under Hypobaric Hypoxia in Japanese Himalayan Climbers
• Efficient Production of Taka-amylase A by Trichoderma viride(Microbiology & Fermentation Industry)
• Isolation and Characterization of a New Polysaccharide-producing Acetobacter sp.
• Activity and Synthesis of β-Galactosidase in Various Lactose Utilizing Bacteria
• Angiotensin I-Converting Enzyme Inhibitory Action of Fish Sauce.
• Detection of Plasmids in Bacillus brevis Species and Introduction of a Plasmid into B. brevis 47, a Protein-producing Bacterium, by Cotransformation
• Structure of an Acidic Polysaccharide from Acetobacter sp. NBI 1022
• Stability of a recombinant plasmid containing .ALPHA.-amylase gene in chemostat.
• Improved Transformation Method for Acetobacter with Plasmid DNA
• Clones with New Phenotypes through Transformation of Bacillus brevis 47, a Protein-producing Bacterium

もっと見る 閉じる

スポンサーリンク