Production of Thermostable Clostridium thermohydrosulfuricum Xylose Isomerase in Bacillus brevis(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• The xylose isomerase gene from the thermophile Clostridium thermohydrosulfuricum has been cloned into Bacillus brevis. Under control of the strong cell wall protein promoter, the gene was efficiently expressed during the early stationary phase of growth, when cell densitied were high. The expressed gene product was a soluble cytoplasmic protein and made up more than 20% of the total cellular protein. A simple heat treatment at 85℃ for 10min gave a virtually pure enzyme. Final isomerase yields were about 0.5g isomerase per liter culture. The purified isomerase has an optimum temperature at 85℃, and an optimum pH around 7. The isomerase is stable at 85℃ for several hours, opening possibilitied for new uses.

• 社団法人日本農芸化学会の論文
• 1991-12-23

著者

• Dekker Koen

  Faculty Of Agriculture Nagoya University:nihon Shokuhin Kako Co. Ltd.

• Udaka S

  Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Udaka Shigezo

  Faculty Of Agriculture Nagoya University:department Of Brewing And Fermentation Tokyo University Of

• Yamagata Hideo

  Department Of Food Science And Technology Facult Of Agriculture Nagoya University

• YAMAGATA Hideo

  Faculty of Agriculture, Nagoya University

• SAKAGUCHI Kenji

  Nihon Shokuhin Kako Co., Ltd.

• Sakaguchi Kenji

  Research Institute For Food Science Kyoto University

• Sakaguchi Kenji

  Nihon Shokuhin Kako Co. Ltd.

• Udaka Shigezo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

関連論文

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