Efficient Production of N-terminally Truncated Biologically Active Human Interleukin-6 by Bacillus brevis
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概要
- 論文の詳細を見る
cDNAs encoding human interleukin 6 (hIL-6) and its variants lacking the N-terminal Pro and Pro-Val-Pro-Pro, respectively, were expressed in Bacillus brevis by using the signal peptide fusion approach. The presence of Pro at the N-terminus of the mature protein hindered the action of the Bacillus brevis signal peptidase. hIL-6 lacking the N-terminal Pro-Val-Pro-Pro was most efficiently secreted in a biologically active form and accumulated in the culture medium to a level of 200 mg per liter, which is the highest level reported for the bacterial secretion of hIL-6.
- 社団法人日本農芸化学会の論文
- 2000-03-23
著者
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EJIMA Daisuke
Pharmaceutical Research Lab., Ajinomoto Co., Inc.
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Kodama Y
Fukuyama Univ. Fukuyama Jpn
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KONISHI Atsushi
Pharmaceutical Research Laboratories, Ajinomoto Co., Inc.
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Kodama Yuko
Pharmaceutical Research Laboratories Ajinomoto Co. Inc.
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Udaka S
Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Udaka Shigezo
Faculty Of Agriculture Nagoya University:department Of Brewing And Fermentation Tokyo University Of
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Ejima D
Ajinomoto Co. Inc. Kawasaki Jpn
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Ejima Daisuke
Pharmaceutical Research Lab. Ajinomoto Co. Inc.
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Yamagata Hideo
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Ohta Toshihiro
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Konishi A
Pharmaceutical Research Laboratories Ajinomoto Co. Inc.
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Konishi Atsushi
Pharmaceutical Research Laboratories Ajinomoto Co. Inc.
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SHIGA Yasuhiro
School of Life Science, Tokyo University of Pharmacy and Life Science
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MAKI Makiko
Graduate School of Bioagricultural Sciences, Nagoya University
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TOKISHITA Shinichi
School of Life Science, Tokyo University of Pharmacy and Life Science
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OKAMOTO Akou
School of Life Science, Tokyo University of Pharmacy and Life Science
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TSUKAGOSHI Norihiro
Graduate School of Bioagricultural Sciences, Nagoya University
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MATSUI Hiroshi
Pharmaceutical Research Laboratories, Ajinomoto Co., Inc.
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Maki Makiko
Graduate School Of Bioagricultural Sciences Nagoya University
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Udaka Shigezo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Okamoto Akou
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Shiga Yasuhiro
School Of Life Science Tokyo University Of Pharmacy And Life Science
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Tsukagoshi N
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Matsui Hiroshi
Pharmaceutical Research Laboratories Ajinomoto Co. Inc.
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