Gene Expression in Bacillus brevis under Control of the Lac Repressor-Operator System from Escherichia coli
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概要
- 論文の詳細を見る
We constructed two inducible plasmid vectors which were different at the replication origin to control the expression of heterologous genes in Bacillus brevis. The Escherichia coli lac repressor gene was expressed by using the promoter region of the cell wall protein (MWP) gene of B. brevis 47. The lac operator was placed downstream from the P2 promoter of the MWP gene. The gene for Bacillus licheniformis α-amylase (BLA) or for human salivary α-amylase (HAMY) was inserted immediately downstream of the lac operator. The production of BLA by B. brevis carrying the inducible plasmids pNU550BLA and pHT550BLA thus constructed was induced 8 and 100-fold, respectively, by the addition of isopropyl-β-D-thio-galactopyranoside (IPTG). The production of HAMY carrying the inducible plasmid pNU550HAMY was induced 6-fold by the addition of IPTG.
- 社団法人日本生物工学会の論文
- 1992-07-25
著者
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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UDAKA SHIGEZO
Department of Fermentation Sciences, Tokyo University of Agriculture
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TAKAGI Hiroaki
Department of Research & Development, Higeta Shoyu Co.
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Takagi Hiroaki
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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Takagi H
Fukui Prefectural Univ. Fukui Jpn
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Udaka S
Faculty Of Applied Bio-science Tokyo University Of Agriculture
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Udaka Shigezo
Department Of Brewing And Fermentation Tokyo University Of Agriculture
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Yamagata Hideo
Department Of Food Science And Technology Facult Of Agriculture Nagoya University
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Udaka Shigezo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Yamagata Hideo
Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University
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