Development of Gene Delivery Systems for the ε-Poly-L-Lysine Producer, Streptomyces albulus(Genetics, Molecular Biology, and Gene Engineering)

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概要

• 論文の詳細を見る

• Streptomyces albulus IFO14147 produces ε-poly-L-lysine (ε-PL), an amino acid homopolymer antibiotic that is used as a food preservative in many countries, including Japan, South Korea, and the United States. To construct an overproducer of the industrially important ε-PL and to develop a deeper understanding of its biosynthetic mechanism, we developed systems for DNA delivery into the S. albulus strain based on both polyethylene glycol-mediated protoplast transformation and intergeneric conjugation from Escherichia coli. The successes of these transformations in particular were accomplished by employing the new cryptic-plasmid-based shuttle vectors constructed in this study. The genetic systems developed here should facilitate a molecular genetic approach to S. albulus.

• 社団法人日本生物工学会の論文
• 2005-06-25

著者

• TAKAGI Hiroshi

  Department of Surgery II, University School of Medicine

• HIRAI TAKANORI

  Komaki Municipal Hospital

• NAKAMORI Shigeru

  Department of Bioscience, Fukui Prefectural University

• Takagi H

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)

• Takagi Hiroshi

  Second Department Of Surgery Nagoya University

• Nakamori Shigeru

  Dept. Biosci. Fukui Pref. Univ.

• Nakamori Shigeru

  Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University

• Takagi H

  Department Of Bioscience Fukui Prefectural University

• Takagi Hiroshi

  Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine

• HAMANO Yoshimitsu

  Department of Biological Sciences, The Nishi Tokyo University

• Nakamori S

  Department Of Bioscience Fukui Prefectural University

• NICCHU INE

  Department of Bioscience, Fukui Prefectural University

• HOSHINO YUSUKE

  Department of Bioscience, Fukui Prefectural University

• KAWAI TAKAHIRO

  Yokohama Research Center

• Takagi H

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Hoshino Yusuke

  Department Of Bioscience Fukui Prefectural University

• Nicchu Ine

  Department Of Bioscience Fukui Prefectural University

• Hamano Yoshimitsu

  Department Of Biological Sciences The Nishi Tokyo University

• Takagi Hiroshi

  Department Of Bioscience Fukui Prefectural University

• Takagi Hiroshi

  Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University

• Hamano Yoshimitsu

  Department of Bioscience, Fukui Prefectural University

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