Partial Purification of Phytase from a Soil Isolate Bacterium, Klebsiella oxytoca MO-3
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概要
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A bacterial strain, Klebsiella oxytoca MO-3,producing phytase (EC 3.1.3.8) was isolated from compost. The phytase was extracted and purified 400-fold and partially characterized. The pH optimum was found to be in the range of 5-6 and the optimum temperature to be 55℃. The phytase is stable at pH 4-8 and no loss of activity upon its incubation at 60℃ for 20 min was detected. The partially purified enzyme was strongly inhibited by NaF, Zn^<2+>, Fe^<2+>, and Cu^<2+> but not inhibited by EDTA or N-ethylmakeimide.
- 社団法人日本生物工学会の論文
- 1997-04-25
著者
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TAKAGI Hiroshi
Department of Surgery II, University School of Medicine
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JAREONKITMONGKOL Saeree
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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NAKAMORI Shigeru
Department of Bioscience, Fukui Prefectural University
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Jareonkitmongkol Saeree
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Jareonkitmongkol Saeree
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University:(present Address)departm
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Watanabe Rika
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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OHYA MASAFUMI
Department of Bioscience, Faculty of Bioscience, Fukui Prefectural University
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Ohya Masafumi
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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Nakamori Shigeru
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Takagi Hiroshi
Department Of Bioscience Fukui Prefectural University
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Takagi Hiroshi
Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University
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