Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli

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概要

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• Transcriptional initiation sites of the ostA gene involved in organic solvent sensitivity in Escherichia coli were found by primer extension analysis. Two transcriptional initiation sites were newly identified at −133 and −48 nucleotides from the initiation codon of ostA, but the previously reported σE-dependent one at −227 could not be detected. No heat-inducible expression of ostA was observed by Northern blotting analysis, indicating that the contribution of σE-dependent transcription was very small if any. σD-Dependent promoter-like sequences were found just upstream of the newly identified transcriptional initiation sites by computer-aided analysis. Deletion analysis of ostA-lacZ fusions demonstrated that these two promoters contributed almost equally to the constitutive expression of the ostA gene.

• 2004-02-23

著者

• Wachi Masaaki

  Department of Bioengineering, Tokyo Institute of Technology

• TAKAGI Hiroshi

  Department of Surgery II, University School of Medicine

• Aono R

  Tokyo Inst. Technol. Yokohama Jpn

• AONO Rikizo

  Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology

• Takagi Hiroshi

  Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Wachi Masaaki

  Department Of Bioengineering Tokyo Institute Of Technology

• Wachi Masaaki

  Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Tech

• Ohtsu Iwao

  Department Of Biological Information Tokyo Institute Of Technology

• Ohtsu Iwao

  Department Of Bioengineering Tokyo Institute Of Technology

• TSUKAGOSHI Norihiko

  Department of Biological Information, Tokyo Institute of Technology

• DOKYU Noriyuki

  Department of Biological Information, Tokyo Institute of Technology

• KAKUDA Nobuto

  Department of Biological Information, Tokyo Institute of Technology

• Tsukagoshi N

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Kakuda Nobuto

  Department Of Biological Information Tokyo Institute Of Technology

• Dokyu Noriyuki

  Department Of Biological Information Tokyo Institute Of Technology

• Takagi Hiroshi

  Department Of Bioscience Fukui Prefectural University

• Tsukagoshi Norihiko

  Department Of Biological Information Tokyo Institute Of Technology

• Aono Rikizo

  Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Techanology

• Takagi Hiroshi

  Department Of Bioscience Faculty Of Bioscience Fukui Prefectural University

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