The Corynebacterium glutamicum NCgl2281 Gene Encoding an RNase E/G Family Endoribonuclease Can Complement the Escherichia coli rng::cat Mutation but Not the rne-1 Mutation
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概要
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The Corynebacterium glutamicum NCgl2281 gene encodes an RNase E/G family endoribonuclease having an additional N-terminal domain of unknown function. In this study, we constructed plasmids expressing the full length (FL) and the N-terminally truncated form (ΔN) of NCgl2281 and examined their complementation ability as to Escherichia coli rng::cat and rne-1 mutations. Both FL- and ΔN-NCgl2281 rescued the defects caused by the rng::cat mutation, i.e., accumulation of 16S rRNA precursor, overproduction of the AdhE protein, and growth inhibition on M9 glucose medium. On the other hand, they did not complement the rne-1 mutation. These results indicate that the C. glutamicum NCgl2281 endoribonuclease is functionally more closely related to the E. coli RNase G than to RNase E.
- 社団法人 日本農芸化学会の論文
著者
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Wachi Masaaki
Department of Bioengineering, Tokyo Institute of Technology
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SAKAI Taro
Department of Surgical and Molecular Pathology, Dokkyo University School of Medicine
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MAEDA Tomoya
Department of Bioengineering, Tokyo Institute of Technology
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