A Role of the cspA Gene Encoding a Mycolyltransferase in the Growth under Alkaline Conditions of Corynebacterium glutamicum
スポンサーリンク
概要
- 論文の詳細を見る
Corynebacterium glutamicum is widely used in the industrial production of amino acids. Producer strains are generated by classical random mutagenesis, and therefore have detrimental characteristics caused by unnecessary mutations. Increased alkali sensitivity is one of those undesired characteristics. We found that one of the laboratory strains, AJ12036ΔcspAΔcspB, showed decreased growth under alkaline conditions. To clarify which mutation is responsible for alkali sensitivity, we constructed mutant strains carrying the ΔcspA and/or ΔcspB mutations from wild-type strain ATCC13869. We found that disruption of cspA encoding a mycolyltransferase alone caused increased alkali sensitivity. The ΔcspA mutant also showed increased susceptibility to ethambutol, penicillin, and rifampicin. Disruption of cspB had no effect on alkali sensitivity or drug sensitivity. These results indicate that the mycolate layer is important for alkali sensitivity as well as drug susceptibility in this bacterium.
著者
-
Takeshita Ryo
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.
-
Ito Hisao
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.
-
Wachi Masaaki
Department of Bioengineering, Tokyo Institute of Technology
関連論文
- A Role of the cspA Gene Encoding a Mycolyltransferase in the Growth under Alkaline Conditions of Corynebacterium glutamicum
- Fluorescent Phospholipid Analogs as Microscopic Probes for Detection of the Mycolic Acid-Containing Layer in Corynebacterium glutamicum : Detecting Alterations in the Mycolic Acid-Containing Layer Following Ethambutol Treatment
- A Corynebacterium glutamicum rnhA recG Double Mutant Showing Lysozyme-sensitivity, Temperature-sensitive Growth, and UV-Sensitivity(Microbiology & Fermentation Technology)
- fcsA29 Mutation is an Allele of polA Gene of Escherichia coli
- Distinct Roles of Two Anaplerotic Pathways in Glutamate Production Induced by Biotin Limitation in Corynebacterium glutamicum(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Transcriptional Analysis of the Escherichia coli mreBCD Genes Responsible for Morphogenesis and Chromosome Segregation
- Transcriptional Analysis of the ostA/imp Gene Involved in Organic Solvent Sensitivity in Escherichia coli
- Isolation and Characterization of the dcw Cluster from the Piezophilic Deep-Sea Bacterium Shewanella violacea
- Anti-infectious Effect of S-Benzylisothiourea Compound A22, Which Inhibits the Actin-Like Protein, MreB, in Shigella flexneri(Microbiology)
- A 4-Aminofurazan Derivative-A189-Inhibits Assembly of Bacterial Cell Division Protein FtsZ In Vitro and In Vivo
- The Corynebacterium glutamicum NCgl2281 Gene Encoding an RNase E/G Family Endoribonuclease Can Complement the Escherichia coli rng::cat Mutation but Not the rne-1 Mutation
- RNase G-Dependent Degradation of the eno mRNA Encoding a Glycolysis Enzyme Enolase in Escherichia coli(Microbiology & Fermentation Technology)
- Mechanism of Inhibition of DNA Gyrase by ES-1273, a Novel DNA Gyrase Inhibitor
- RNase ES of Streptomyces coelicolor A3(2) Can Complement the rne and rng Mutations in Escherichia coli(Microbiology & Fermentation Technology)
- Structure-Activity Relationship Study of the Bacterial Actin-Like Protein MreB Inhibitors : Effects of Substitution of Benzyl Group in S-Benzylisothiourea
- Structure-Activity Relationship Study of the Bacterial Actin-Like Protein MreB Inhibitors: Effects of Substitution of Benzyl Group in S-Benzylisothiourea
- Isolation of a New Antibiotic, Alaremycin, Structurally Related to 5-Aminolevulinic Acid from Streptomyces sp. A012304
- The Corynebacterium glutamicum NCg12281 Gene Encoding an RNase E/G Family Endoribonuclease Can Complement the Escherichia coli rng :: cat Mutation but Not the rne-1 Mutation
- Cytoplasmic Acidification May Occur in High-Pressure Carbon Dioxide-Treated Escherichia coli K12
- Characterization of a Unique Mutant lysE Gene, Originating from Corynebacterium glutamicum, Encoding a Product That Induces L-Lysine Production in Methylophilus methylotrophus
- A Role of the cspA Gene Encoding a Mycolyltransferase in the Growth under Alkaline Conditions of Corynebacterium glutamicum