Cytoplasmic Acidification May Occur in High-Pressure Carbon Dioxide-Treated Escherichia coli K12

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概要

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• While studying the mechanism by which high-pressure carbon dioxide treatment (HCT) inactivates bacteria, we found that the efficiency of DNA recovery via phenol extraction was extraordinarily low from E. coli K12 cells that had been subjected to HCT. DAPI staining of the treated cells, however, revealed that nuclear DNA was present. Most DNA from the cells subjected to HCT was probably caught in the denatured protein layer during phenol extraction. The efficiency of DNA recovery from proteinase-treated crude extracts from cells subjected to HCT was high. Crude extracts of E. coli K12 cells that had not undergone HCT were intentionally acidified with acetic acid to pH 5.2 to cause acidic coagulation of cytoplasmic proteins. The efficiency of DNA recovery from the acidified extracts was low. These results suggest that in cells subjected to HCT, cytoplasmic pH is reduced to around pH 5.2, and that nuclear DNA becomes entangled in coagulated cytoplasmic proteins. Acidification of the cytoplasm might be the primary mechanism by which HCT inactivates bacteria.

• 社団法人 日本農芸化学会の論文
• 2007-10-23

著者

• Wachi Masaaki

  Department of Bioengineering, Tokyo Institute of Technology

• Yamasaki M

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And

• Yamashita Masahiro

  Department Of Bioengineering Tokyo Institute Of Technology

• Watanabe Taisuke

  RIKEN (The Institute of Physical and Chemical Research)

• WATANABE Taisuke

  Department of Biotechnology, The University of Tokyo

• Yamasaki Makari

  Advanced Research Institute For The Science And Humanities Nihon University

• Yamasaki Makari

  Department Of Biotechnology University Of Tokyo

• Wachi Masaaki

  Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Tech

• FURUKAWA Soichi

  Department of Food Science and Technology, College of Bioresource Sciences, Nihon University

• OGIHARA Hirokazu

  Department of Food Science and Technology, College of Bioresource Sciences, Nihon University

• Furukawa S

  Kyushu Univ. Fukuoka Jpn

• Furukawa Soichi

  Laboratory Of Food Process Engineering Division Of Bioresource And Bioenvironmental Sciences Graduat

• KAWARAI Taketo

  Department of Bacteriology, National Institute of Infectious Diseases

• Kawarai Taketo

  Department Of Food Science And Technology College Of Bioresource Sciences Nihon University:(present

• Kawarai Taketo

  Department Of Bacteriology National Institute Of Infectious Diseases

• Ogihara Hirokazu

  Department Of Food Science And Technology College Of Bioresource Science Nihon University

• Watanabe Taisuke

  Department Of Food Science And Technology College Of Bioresource Sciences Nihon University

• Ogihara H

  Department Of Food Bioscience And Biotechnology College Of Bioresource Sciences Nihon University

• Ogihara Hirokazu

  Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University

• Furukawa Soichi

  Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University

• Watanabe Taisuke

  Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus

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