Amplification of an Integration Plasmid Introduced into the tmr A Amplification Unit of A Bacillus subtitis Tunicamycin-resistant Mutant
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概要
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An integration plasmid for Bacillus subtilis, pTUE128 (8.2kb) which contained the amyE-bla fused gene, the cat gene, and the DNA replication origin of the E. coli plasmid μBR322, was constructed and was inserted into the amyE region of the chromosome of a tunicamycin-resistant B. subtilis mutant (tmr A7 mutation). After cultivation of the transformants with 100μg/ml of chloramphenicol and/or 10μg/ml of tunicamycin, amplification of pTUE128 was analyzed by the increase of β-lactamase activity, the gene for which was included in pTUE128 and of α-amylase activity from the chromosomal gene, and by the method of DNA-DNA hybridization. This was done using the 24.5 kb amplification unit of tmr A7 either by the addition of the high concentration of chloramphenicol or by the addition of tunicamycin.
- 社団法人 日本農芸化学会の論文
著者
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Yamasaki M
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And
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YAMASAKI Makari
Department of Agricultural Chemistry, the University of Tokyo
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YODA Koji
Department of Biotechnology, University of Tokyo
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Yoda K
Univ. Tokyo Tokyo Jpn
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YAMANE Kunio
Institute of Biological Sciences, University of Tsukuba
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Yamane K
Univ. Tsukuba
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Yamane Kunio
Institute Of Biological Sciences University Of Tsukuba
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Nakamura Junji
Institute Of Materials Science University Of Tsukuba
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Yoda Koji
Department Of Agricultural Chemistry The University Of Tokyo
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Yamasaki Makari
Department Of Agricultural Chemistry The University Of Tokyo
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Nakamura Junji
Institute Of Materials Science Graduate School Of Pure And Applied Science University Of Tsukuba
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NAKAMURA Junji
Institute of Biological Sciences, University of Tsukuba
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YAMANE Kunio
Institute of Applied Microbiology, The University of Tokyo
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