Expression of the β-Cyclodextrin Glucanotransferase Gene of an Alkalophilic Bacillus sp. #1011 in Escherichia coli Cells and Characterization of the Synthesized Enzyme(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
To express efficiently the gene for extracellular β-cyclodextrin glucanotransferase(β-CGTase) of an alkalophilic Bacillus sp. #1011 using the E. coli promoters (tac, tip and P L promoters), three DNA fragments starting from the nucleotide positions +1, -18, and -48 of the translation initiation site of the gene were prepared and they were fused with the promoters. The maximum production of the enzyme, which was located mainly (90%) in the periplasm of the E, coli strain, was observed in the combination of the tip promoter and the β-CGTase gene starting from the -48 nucleotide position in the presence of the inducer, IAA. The production of the enzyme was increased to 5.5 times that by the E. coli harboring the original plasmid and to approximately 3 times higher than the extracellular production of the enzyme by the parental Bacillus sp. #1011. The properties including the stability and optimum in the high pH range (pH9) of the extracellular β-CGTase from the alkalophilic Bacillus was conserved in the periplasmic enzymes of the E. coli cells.
- 社団法人日本農芸化学会の論文
- 1990-03-23
著者
-
YAMANE Kunio
Institute of Biological Sciences, University of Tsukuba
-
Yamane Kunio
Institute Of Biological Sciences University Of Tsukuba
-
TAKANO Toshiya
National Food Research Institute
-
Ishii Yasumasa
Central Laboratory Oji-cornstarch Co. Ltd.
-
KIMURA Kenji
Institute of Biological Sciences, University of Tsukuba
-
KATAOKA Shinsuke
Institute of Biological Sciences, University of Tsukuba
-
Kataoka Shinsuke
Institute Of Biological Sciences University Of Tsukuba
-
Kimura Kenji
Institute Of Biological Sciences University Of Tsukuba:central Laboratory Oji-cornstarch Co. Ltd.
-
YAMANE Kunio
Institute of Applied Microbiology, The University of Tokyo
関連論文
- The Gene Responsible for Tunicamycin Resistance, tmrB, in Bacillus subtilis(Microbilolgy & Fermentation Industry)
- Gene Amplification of the amy E-tmrB Region in Bacillus subtilis
- Amplification of an Integration Plasmid Introduced into the tmr A Amplification Unit of A Bacillus subtitis Tunicamycin-resistant Mutant
- Mutational Site in Tunicamycin-resistant Mutation tmrB8 of Bacillus subtilis as Revealed at the Nucleotide Sequence Level(Microbiology & Fermentation Industry)
- recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)
- Maturation of an NH_2-Terminallly Extended Thermostable α-Amylase in Bacillus subtilis : A Possible Mechanism Examined by in Vitro Experiments
- Enhancing Effect of Bacillus subtilis Ffh, a Homologue of the SRP54 Subunit of the Mammalian Signal Recognition Particle, on the Binding of SecA to Precursors of Secretory Proteins In Vitro
- Effects of Essential Carbohydrate/Aromatic Stacking Interaction with Tyr100 and Phe259 on Substrate Binding of Cyclodextrin Glycosyltransferase from Alkalophilic Bacillus sp. 1011
- Crystal Structure of Cyclodextrin Glucanotransferase from Alkalophilic Bacillus sp. 1011 Complexed with 1-Deoxynojirimycin at 2.0 A Resolution
- Crystal Structure of Alkalophilic Asparagine 233-Replaced Cyclodextrin Glucanotransferase Complexed with an Inhibitor, Acarbose, at 2.0 A Resolution
- Molecular Cloning and Analysis of Nucleotide Sequence of the Bacillus subtilis lysA Gene Region Using B. subtilis Phage Vectors and a Multi-copy Plasmid, pUB110(Biological Chemistry)
- Expression of the Cyclodextrin Glucanotransferase Gene of Bacillus macerans in Bacillus brevis
- Protein Traffic for Secretion and Related Machinery of Bacillus subtilis
- Cloning and Nucleotide Sequence of the Bacillus subtilis K trpB Gene Encoding Tryptophan Synthase β-Subunit
- Expression of the β-Cyclodextrin Glucanotransferase Gene of an Alkalophilic Bacillus sp. #1011 in Escherichia coli Cells and Characterization of the Synthesized Enzyme(Biological Chemistry)
- The Rapid Degradation of Mutant SecA Protein in the Bacillus subtilis secA341 (ts) Mutant Causes a Protein Translocation Defect in the Cell
- Nucleotide Sequence of the Shikimate Kinase gene (aroI) of Bacillus subtilis
- Cloning and Nucleotide Sequence of the Maltopentaose-forming Amylase Gene from Pseudomonas sp. KO-8940
- Cloning of Thermostable α-Amylase Gene Using Bacillus subtilis Phage ρll as a Vector
- Cloning of Exo-maltotetraohydrolase Gene from Pseudomonas saccharophila in Escherichia coli
- Cloning and Identification of an Exogenous Thermostable a-Amylase Gene residing on the Bacillus subtilis Chromosome
- Stepwise genetic transformation of Bacillus subtilis with enhancement of productivity of .ALPHA.-amylase.
- Instability of a Chimeric Plasmid Containing Bacillus subtilis aroI+ tmrB Genes, pBR322 and pUBHO, during B. subtilis Transformation
- Genetic and Enzymatic Studies on Thermostable α-Amylase of Bacillus subtilis Carrying the Amylase Gene from a Thermophilic Bacterium
- Enhancing Effect of Bacillus subtilis Ffh, a Homologue of the SRP54 Subunit of the Mammalian Signal Recognition Particle, on the Binding of SecA to Precursors of Secretory Proteins In Vitro.