Protein Traffic for Secretion and Related Machinery of Bacillus subtilis
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概要
- 論文の詳細を見る
Gram-positive sporulating Bacillus subtilis secretes high levels of protein. Its complete genome sequence, published in 1997, encodes 4,106 proteins. Bioinformatic searches have predicted that about half of all B. subtilis proteins are related to the cell membrane through export to the extracellular medium, insertion, and attachment. Key features of the B. subtilis protein secretion machinery are the absence of an Escherichia coli SecB homolog and the presence of an SRP (signal recognition particle) that is structurally rather similar to human SRP. In addition, B. subtilis contains five type I signal peptidases (SipS, T, U, V, and W). Our in vitro assay system indicated that co-operation between the SRP–protein targeting system to the cell membrane and the Sec protein translocation machinery across the cytoplasmic membrane constitutes the major protein secretion pathway in B. subtilis. Furthermore, the function of the SRP–Sec pathway in protein localization to the cell membrane and spore was analyzed.
- 社団法人 日本農芸化学会の論文
- 2004-10-23
著者
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YAMANE Kunio
Institute of Biological Sciences, University of Tsukuba
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Yamane Kunio
Institute Of Biological Sciences University Of Tsukuba
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BUNAI Keigo
Institute of Biological Sciences, University of Tsukuba
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KAKESHITA Hiroshi
Institute of Biological Sciences, University of Tsukuba
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Bunai Keigo
Institute Of Biological Sciences University Of Tsukuba
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Kakeshita Hiroshi
Institute Of Biological Sciences University Of Tsukuba
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YAMANE Kunio
Institute of Applied Microbiology, The University of Tokyo
関連論文
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- Molecular Cloning and Analysis of Nucleotide Sequence of the Bacillus subtilis lysA Gene Region Using B. subtilis Phage Vectors and a Multi-copy Plasmid, pUB110(Biological Chemistry)
- Expression of the Cyclodextrin Glucanotransferase Gene of Bacillus macerans in Bacillus brevis
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- Expression of the β-Cyclodextrin Glucanotransferase Gene of an Alkalophilic Bacillus sp. #1011 in Escherichia coli Cells and Characterization of the Synthesized Enzyme(Biological Chemistry)
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