Molecular Cloning and Analysis of Nucleotide Sequence of the Bacillus subtilis lysA Gene Region Using B. subtilis Phage Vectors and a Multi-copy Plasmid, pUB110(Biological Chemistry)
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概要
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A 3.8-kb EcoRl-fragment containing the lysA gene [diaminopimelate (DAP) decarboxylase] of Bacillus subtilis has been cloned into B. subtilis phage Φ105 and its nucleotides sequenced. The nucleotide sequence of a 3,762 by stretch contained three open reading frames (ORFI, ORF2, and ORF3) in one orientation and another open reading frame (ORF4) in the opposite orientation. ORF2 coded for the IysA gene based on the complementation of a B. suhtilis lys auxotroph and on the fact that the predicted amino acid sequence (440 amino acids with a molecular weight of 48,876) of ORF2 shared a 29.7%, 38.3%, and 32.9% identity with the sequences of Escherichia coli, Corynehacterium glutamicum and Pseudomonas aeruginosa lysA genes, respectively. ORF1, 011173, and ORF4 did not correspond to E. coli lysR. Based on the comparison of the B. suhtilis lysA sequence with a sequence of the DAP-decarboxylase gene cloned into pUB110 (Yamamoto et al., Nucleic Acids Res., 17, 10105 (1989)), it was found that the lys gene in the plasmid was fused with the dnaN gene in its COOH-terminal region.
- 社団法人日本農芸化学会の論文
- 1991-06-23
著者
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YAMANE Kunio
Institute of Biological Sciences, University of Tsukuba
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Yamane Kunio
Institute Of Biological Sciences University Of Tsukuba
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YAMAMOTO Junya
Institute of Biological Sciences, University of Tsukuba
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SHIMIZU Mikio
Zen-noh Institute of Animal Health
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Yamamoto Junya
Institute Of Biological Sciences University Of Tsukuba:zen-noh Institute Of Animal Health
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YAMANE Kunio
Institute of Applied Microbiology, The University of Tokyo
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