recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)

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概要

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• A recombinant Bacillus subtilis phage, ρ11-AA248, contains the tmrA7-amyR2-amyE^+-tmrB^+-aroI^+ region of the B. subtilis N7 chromosome on a 22.4kb DNA fragment. The amyE^+-tmrB^+ gene region in the phage genome of the B. subtilis 207-21 transductants by ρ11-AA248 was amplified to approximately 10 copies after cultivation in the presence of tunicamycin (10μg/ml) and to two copies without tunicamycin. The amplification of the gene region caused hyper-production of extracellular α-amylase. In contrast, no amplification of the gene region was detected in the transductants of B. subtilis 207-25, a recE-deficient derivative of 207-21 strain.

• 社団法人日本農芸化学会の論文
• 1986-07-23

著者

• Yamasaki M

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And

• Yamashita Masahiro

  Department Of Bioengineering Tokyo Institute Of Technology

• YAMASAKI Makari

  Department of Agricultural Chemistry, the University of Tokyo

• YODA Koji

  Department of Biotechnology, University of Tokyo

• Yoda K

  Univ. Tokyo Tokyo Jpn

• HARADA Shigeyoshi

  Department of Agricultural Chemistry, University of Tokyo

• MORI Masaki

  Department of Agricultural Chemistry, University of Tokyo

• TANIMOTO Akihiko

  Department of Agricultural Chemistry, University of Tokyo

• FURUSATO Takashi

  Institute of Biological Sciences, University of Tsukuba

• YAMANE Kunio

  Institute of Biological Sciences, University of Tsukuba

• TAMURA Gakuzo

  Department of Agricultural Chemistry, University of Tokyo

• Yamane K

  Univ. Tsukuba

• Tamura G

  Department Of Agricultural Chemistry The University Of Tokyo

• HASHIGUCHI Ken-ichi

  Department of Agricultural Chemistry, The University of Tokyo

• Mori M

  Ajinomoto Co. Inc. Kawasaki Jpn

• Yamane Kunio

  Institute Of Biological Sciences University Of Tsukuba

• Tamura Gakuzo

  Depart Ment Qf Agricultural Chemistry The University Of Tokyo

• Yoda Koji

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamasaki Makari

  Department Of Agricultural Chemistry The University Of Tokyo

• FUWA TORU

  Institute for Biotechnology Research, Wakunaga Pharmaceutical Co. Ltd.,

• Tamura Gakuzo

  Nuclear Receptor Institute Ksp

• Hashiguchi Ken-ichi

  Department Of Agricultural Chemistry The University Of Tokyo

• MORI Masaaki

  Life Science Research Center, Shiseido Co., Ltd.

• Mori M

  Institute For Cosmic Ray Research University Ot Tokyo

• Fuwa T

  Institute For Biotechnology Research Wakunaga Pharmaceutical Co. Ltd.

• Tanimoto Akihiko

  Department Of Agricultural Chemistry The University Of Tokyo:(present Office)gyosei High School

• Harada Shigeyoshi

  Department Of Agricultural Chemistry The University Of Tokyo

• Yoda K

  Institute Of Biological Sciences University Of Tsukuba

• YAMANE Kunio

  Institute of Applied Microbiology, The University of Tokyo

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