Molecular Characterization of a Novel Yeast Cell-wall Acid Phosphatase Cloned from Kluyveromyces marxianus

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概要

• 論文の詳細を見る

• A novel Kluyveromyces marxianus gene that encodes an acid phosphatase, Pho610,was cloned in Saccharomyces cerevisiae. The deduced amino acid sequence was distinct from S. cerevisiae phosphatases but similar to some fungal enzymes. A peculiar feature of the sequence is that it has hydrophobic stretches both at the N- and C-termini, which is a characteristic of the precursors of glycosylphosphatidylinositol (GPI)-anchored proteins. When the gene was expressed in S. cerevisiae, the active enzyme was recovered in the periplasmic fraction by glucanase digestion. The Pho610 polypeptide was highly glycosylated and a significant portion was covalently linked to the cell-wall glucan. The enzyme was secreted when the C-terminal region was truncated to remove the GPI signal. Therefore, Pho610 is a novel cell-wall protein having an enzyme activity.

• 社団法人日本農芸化学会の論文
• 2000-01-23

著者

• Yamasaki M

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And

• Yamashita Masahiro

  Department Of Bioengineering Tokyo Institute Of Technology

• YAMASAKI Makari

  Department of Agricultural Chemistry, the University of Tokyo

• YODA Koji

  Department of Biotechnology, University of Tokyo

• Yoda K

  Univ. Tokyo Tokyo Jpn

• NODA Yoichi

  Department of Biotechnology, University of Tokyo

• LIN Ying

  Department of Preventive Medicine, Nagoya University, School of Medicine

• HASHIMOTO Hitoshi

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University

• Yamane K

  Univ. Tsukuba

• Noda Y

  Univ. Tokyo Tokyo Jpn

• Noda Y

  Department Of Gynecology And Obstetrics Kyoto University Faculty Of Medicine

• Lin Y

  Department Of Biotechnology The University Of Tokyo

• Lin Ying

  Department Of Pediatrics Faculty Of Medicine Kyoto University

• Yoda Koji

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamasaki Makari

  Advanced Research Institute For The Science And Humanities Nihon University

• Yamasaki Makari

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamasaki Makari

  Department Of Biotechnology University Of Tokyo

• KAWADA Tsuyoshi

  Department of Biotechnology, The University of Tokyo

• KAIBARA Chiaki

  Department of Biotechnology, The University of Tokyo

• TOMISHIGE Nario

  Department of Biotechnology, The University of Tokyo

• KO JungHwan

  Department of Biotechnology, the University of Tokyo

• NAGAMATSU Takuya

  Department of Biotechnology, the University of Tokyo

• Hashimoto H

  Kyoto Inst. Technol. Kyoto Jpn

• Ko Junghwan

  Department Of Biotechnology The University Of Tokyo

• Kaibara Chiaki

  Department Of Biotechnology The University Of Tokyo

• Noda Yoichi

  Department Of Biotechnology University Of Tokyo

• Kawada T

  Department Of Biotechnology The University Of Tokyo

• Lin Ying

  Department Of Electrical Engineering National Cheng Kung University

• Hashimoto H

  Department Of Biotechnology The University Of Tokyo

• Hashimoto Hitoshi

  Department Of Marine Science And Technology Tokai University

• Yoda K

  Institute Of Biological Sciences University Of Tsukuba

• Tomishige Nario

  Department Of Biotechnology The University Of Tokyo

• Nagamatsu Takuya

  Department Of Biotechnology The University Of Tokyo

• Hashimoto Hitoshi

  Department Of Biotechnology The University Of Tokyo

• Ko Jung-Hwan

  Department of Biotechnology, the University of Tokyo

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