Molecular Cloning of a Gene, DHS1, Which Complements a Drug-hypersensitive Mutation of the Yeast Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
We isolated a Saccharomyces cerevisiae mutant, CH108, which shows hypersensitivity to cycloheximide, bleomycin, actinomycin D, 5-fluorouracil, and several other antibiotics. The mutant cells showed irregular shapes with swelling and were also hypersensitive to Zymolyase digestion. The mutant cell wall was swelled and twice as thick as the wild type cell wall. These defects were completely reversed by introduction of a genomic clone on a single-copy vector. Restriction analysis and sequencing showed that the responsible gene, DHS1, encodes a novel hydrophilic polypeptide of 483 amino acids.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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Yamasaki M
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And
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YAMASAKI Makari
Department of Agricultural Chemistry, the University of Tokyo
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YODA Koji
Department of Biotechnology, University of Tokyo
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Yoda K
Univ. Tokyo Tokyo Jpn
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Yamane K
Univ. Tsukuba
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Yoda Koji
Department Of Agricultural Chemistry The University Of Tokyo
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Yamasaki Makari
Advanced Research Institute For The Science And Humanities Nihon University
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Yamasaki Makari
Department Of Agricultural Chemistry The University Of Tokyo
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Lee Sik
Department of Agricultural Chemistry, The University of Tokyo
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Shimizu Jiro
Department of Agricultural Chemistry, The University of Tokyo
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Shimizu Jiro
Department Of Biotechnology The University Of Tokyo
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Shimizu Jiro
Department Of Agricultural Chemistry The University Of Tokyo
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Yoda K
Institute Of Biological Sciences University Of Tsukuba
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Lee Sik
Department Of Agricultural Chemistry The University Of Tokyo
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