E-15, a Novel Polysaccharide Mitogen from Nocardia Specific for B Cells(Microbiology & Fermentation Industry)

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概要

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• A novel mitogen, E-15, which induced blastogenesis of mouse aplenocytes, was purified from the culture filtrate of an actinomycete through ethanol precipitaion, anion and cation exchange column chromatography, and gel filtration. The producing organism was identified as Nocardia asteroides. Spectronic study demonstrated that it was a polysaccharide. Acid hydrolysis of E-15 yielded glucose and glucosamine. The active substance was eluted at the molecular mass between 90kDa and 750 kDa on gel filtration. E-15 induced a mitogenic response of mouse splenocytes above 1μg/ml and its potency of induction was superior to a lipopolysaccharide that is known to be a polyclonal B cell-specific mitogen. It showed no toxicity up to 100μg/ml. The cell surface phenotypes of the blast cells induced by E-15 were analyzed by flow cytometry; they had surface immunoglobulins but no Lyt-2 antigen. Thus it was suggested that E-15 was a B-cell-specific mitogen.

• 1991-12-23

著者

• MUNEMURA Kazuko

  Department of Agricultural Chemistry, the University of Tokyo

• MAGAE Junji

  Department of Bioengineering, Tokyo Institute of Technology

• NAGAI Kazuo

  Department of Bioengineering, Tokyo Institute of Technology

• Yamamoto Masakuni

  Department of Agricultural Chemistry, The University of Tokyo

• Kurisaki Tomohiro

  Department of Agricultural Chemistry, The University of Tokyo

• Yamasaki Makari

  Department Of Agricultural Chemistry The University Of Tokyo

• Nagai Kazuo

  Department Of Applied Biological Chemistry Chubu University

• Munemura Kazuko

  Department Of Agricultural Chemistry The University Of Tokyo

• Isono Kiyoshi

  Riken The Institute Of Physical And Chemical Research

• Uramoto Masakazu

  Riken (the Institute Of Physical And Chemical Research)

• Kusakabe Hiroo

  Riken The Institute Of Physical And Chemical Research

• HAYASHI Midori

  Department of Agricultural Chemistry, The University of Tokyo

• KOBAYASHI Kyuichi

  Department of Agricultural Chemistry, The University of Tokyo

• NAKAMURA Akito

  Department of Agricultural Chemistry, The University of Tokyo

• ISONO Kiyoshi

  RIKEN Institute

• KUSAKABE Hiroo

  RIKEN Institute

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