Insulin-Dependent Adipogenesis in Stromal ST2 Cells Derived from Murine Bone Marrow(Biochemistry & Molecular Biology)

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概要

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• ST2 cells, a cloned stromal-cell line from mouse bone marrow have the phenotypes of osteoblasts and hematopoietic supporting cells, but little is known about the adipogenesis in this cell line. We found that treatment of ST2 cells with a cocktail, a combination of insulin, dexamethasone (DEX) and 3-isobutyl-1-methylxanthine (IBMX), induced adipocyte differentiation. The cells were adipocytic, as seen by the accumulation of triglycerides and with lipid droplets stained with Oil Red O. Expressions of the adipogenic transcriptional factors peroxisome-proliferator-activated receptor γ and CCAAT-enhancer binding protein α were stimulated, triggering the expression of the late adipocyte-specific marker α-glycerophosphate-3-dehydrogenase. Unlike another bone marrow stromal cell line PA6, ST2 cells responded to the adipogenic effects of insulin, as do the extramedullary preadipocytes 3T3-L1. Like PA6 and 3T3-L1 cells, adipogenesis in ST2 cells was inhibited by 1, 25-dihydroxyvitamin D3, retinoic acid, tumour necrosis factor α, and transforming growth factor β. Therefore, ST2 cells can differentiate into osteoblasts, adipocytes, and hematopoietic supporting cells, in which the process of adipogenesis differs from that of bone marrow preadipocytes PA6, and resembles that of the extramedullary preadipocytes 3T3-L1.

• 社団法人日本農芸化学会の論文
• 2003-02-23

著者

• Nagai Kazuo

  Department Of Applied Biological Chemistry Chubu University

• Nagai Kazuo

  Research Institute for Biological Functions, Chubu University

• Woo Je-tae

  Department Of Bioscience And Biochemistry College Of Bioscience And Biotechnology Chubu University

• Woo Je-tae

  Department Of Applied Biological Science Tokyo Noko University

• Nagai Kazuo

  Department Of Biological Chemistry Chubu University

• DING Jin

  Department of Bioscience and Biochemistry, College of Bioscience and Biotechnology, Chubu University

• Ding Jin

  Department Of Bioscience And Biochemistry College Of Bioscience And Biotechnology Chubu University

• Nagai Kazuo

  Research Institute For Biological Functions Chubu University

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