Immunochemical Detection of GTP-binding Protein in Cephalopod Photoreceptors by Anti-peptide Antibodies
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概要
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We prepared polyclonal antibodies (Pab) against the following peptides : partial sequences of bovine transducin α subunit including the ADP-ribosylation sites sensitive to cholera toxin (CTX) and pertussis toxin (PTX) and the N-terminus of Drosophila GTP-binding protein qα (DGqN) ; Pab CTX, Pab PTX and Pab DGqN, respectively. These antibodies were specific to the peptides used as antigen and no crossreactivity was observed. Pab CTX and Pab PTX reacted with bovine transducin α subunit and the reactivity was lost by preincubation with the specific antigen peptide. Proteins of 41-42 kDa in octopus and squid photoreceptor membranes were recognized by Pab DGqN but not by Pab CTX or Pab PTX. Anti-α antibody (GA/1) reacted with the same bands as Pab DGqN recognized. These results suggest that the major GTP-binding protein in cephalopod photoreceptors is a Gq-type, similar to Drosophila Gq.
- 社団法人日本動物学会の論文
- 1993-06-15
著者
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Nagai Kazuo
Department Of Applied Biological Chemistry Chubu University
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SUZUKI Tatsuo
Department of Research Planning, Kitasato Institute Hospital
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YOSHIHARA Kazuo
Suntory Institute for Bioorganic Research
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Kito Yuji
Department Of Biology Faculty Of Science Osaka University
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Nagai K
Teikyo Univ. School Of Medicine Tokyo Jpn
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Narita Kinya
Department Of Biology Faculty Of Science Osaka University
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Yoshihara K
Suntory Inst. Bioorganic Res. Osaka Jpn
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Suzuki Tatsuo
Department Of Neuroplasticity Research Center On Aging And Adaptation Shinshu University School Of M
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Suzuki Tatsuo
Department Of Biochemistry Nagoya City University Medical School
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