Estimation of pH and the Number of Lytic Granules in a CD8^+ CTL Clone Treated with an Inhibitor of Vacuolar Type H^+-ATPase, Concanamycin A
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概要
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A vacuolar type H^+-ATPase inhibitor, concanamycin A (CMA), raised the pH and induced morphologic changes such as vacuolation in lytic granules in the CD8^+ cytotoxic T lymphocyte clone OE4. Here we measured the pH in the lytic granules present in OE4 by immunoelectron microscopic observation using 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP) as a pH probe. In control OE4, only the peripheral region, but not cores, were heavily stained with DAMP. Exposure to 100 nM CMA for 60 min raised the pH in the lytic granules from 5.7±0.2 to 6.8±0.2. Furthermore, the number of lytic granules in OE4 was estimated under phase contrast microscopy after Wright staining. About 20 lytic granules in average were detected in control cells, while CMA induced vacuolation and decreased their number by 20 to 30%.
- 社団法人日本農芸化学会の論文
- 1996-10-23
著者
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KATAOKA Takao
Department of Bioengineering, Tokyo Institute of Technology
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NAGAI Kazuo
Department of Bioengineering, Tokyo Institute of Technology
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Nagai Kazuo
Department Of Applied Biological Chemistry Chubu University
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Kataoka Takao
Department Of Bioengineering Tokyo Institute Of Technology
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Kondo Shunzo
Electron Microscopy Section Mitsubishi Kasei Institute Of Life Sciences
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SATO Michio
Electron Microscopy Section, Mitsubishi Kasei Institute of Life Sciences
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Sato Michio
Electron Microscopy Section Mitsubishi Kasei Institute Of Life Sciences
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Kataoka Takao
Department Of Applied Biology Kyoto Institute Of Technology
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