Interaction of Chicken Gizzard Smooth Muscle Calponin with Brain Microtubules.
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概要
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Calponin, a major actin-, tropomyosin-, and calmodulin-binding protein in smooth muscle, interacted with tubulin, a main constituent of microtubules, in a concentration-dependent fashion in vitro. The apparent Kd value of calponin to tubulin was calculated to be 5.2μM with 2mol of calponin maximally bound per 1mol of tubulin. At low ionic strength, tubulin bound to calponin immobilized on Sepharose 4B, and the bound protein was released at about 270mM NaCl. Chemical cross-linking experiments showed that a 1:1 molar covalent complex of calponin and tubulin was produced. The amount of calponin bound to microtubules decreased with increasing ionic strength or Ca2+ concentration. The addition of calmodulin or S100 to the mixture of calponin and microtubule proteins caused the removal of calponin from microtubules in the presence of Ca2+, but not in the presence of EGTA. Calponin-related proteins including tropomyosin, SM22, and caldesmon had little effect on the calponin binding to microtubules, whereas MAP2 inhibited the binding. Interestingly, there was little, if any, effect of mycalolide B-treated actin on the binding of calponin to microtubules. Furthermore, only about 20% of calponin-F-actin interaction was inhibited in the presence of an excess amount of tubulin (4mol per mol of calponin), indicating that tubulin binds to calponin at a different site from that of actin. Compared with MAP2, calponin had little effect on microtubule polymerization.
- 社団法人 日本生化学会の論文
著者
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Hamamoto Masateru
Department Of Imagination Science (kansai Engineering) Faculty Of Textile Science And Technology Shi
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Hiromori Tsuneyoshi
Department Of Imagination Science (kansai Engineering) Faculty Of Textile Science And Technology Shi
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Fujii Toshihiro
Department Of Biological Science School Of Science Nagoya University Nagoya
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Suzuki Tatsuo
Department Of Biochemistry Nagoya City University Medical School
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Hiromori Tsuneyoshi
Department of Imagination Science (Kansei Engineering), Faculty of Textile Science and Technology, Shinshu University
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