Retinal and 3-Dehydroretinal in the Egg of the Clawed Toad, Xenopus laevis : Cell Biology and Biochemistry

概要

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Retinals in the egg of the clawed toad, Xenopus laevis, were extracted and the composition of the geometric isomers was analysed by using high-performance liquid chromatography. Nearly equal amounts of retinal (all-trans and 13-cis) and 3-dehydroretinal (all-trans and 13-cis) were present at the total quantity about 50pmol/egg. In the eggs of other frogs, Rhacophorus schlegelii and Rhacophorus arboreus, only retinal was contained at the quantity about 50pmol/egg. Retinyl ester and carotenoids in the Xenopus eggs were scarce, and retinols, 3-hydroxyretinals or 11-cis isomers of retinals were undetectable in them. Retinals in the Xenopus eggs were suggested to be present in a protein-bound form through the Schiff base linkage, for after reduction by NaBH4 neither retinol nor retinyl-product could be found in the organic solvent layer. The metabolic pathways forming the egg retinals are discussed on the basis of retinyl ester and carotenoids found in the Xenopus ovary.
社団法人日本動物学会の論文
1987-06-15