Localization of Retinal Proteins in the Stalk and Dorsal Eyes of the Marine Gastropod, Onchidium : Physiology
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概要
- 論文の詳細を見る
Onchidium possesses stalk eye (SE) and dorsal eye (DE) which comprise part of a unique multiple photoreceptive system. The retina of SE consists of rhabdomeric-type visual cells, where as the DE contains two types of photoreceptor cells ; ciliary-type cells in the retina and rhabdomeric-type cells in the lens. High-performance liquid chromatography (HPLC) analyses revealed the presence of 11-cis-retinal as well as all-trans-retinal in both eyes. The amount of retinal of one DE (0.17pmol) is far less than that in one SE (0.41pmol) in the dark-adapted Onchidium. In the dark-adapted SE, the amount of all-transretinal was higher than that of 11-cis-retinal. This finding is consistent with the presence of photic vesicles, including retinochrome, in rhabdomeric-type visual cells. In contrast, a higher amount of 11-cis-retinal than all-trans-retinal was present in dark-adapted DE, although this was decrease dinlight-adapted DE. Upon UV irradiation following treatment with sodium borohydride (NaBH_4), the fluorescence (derived from retinochrome) was observed in the somatic layer of SE. Additional fluorescence (due to rhodopsin) was observed in the villous layer upon treatment with NaBH_4 after denaturation. However, only weak, obscure fluorescence of retinyl proteins was observed in the DE, not in a specific but an indefinite area on treatment with NaBH_4 with or without denaturation. With fluorescence histochemistry, the localization of rhodopsin and retinochrome was confirmed at specific regions in the retina of the SE, whereas no distinct localization of these photopigments in DE was demonstrated. The amount of retinal to detect the fluorescence may be too low in the DE, orphotopigments of DE may differ in chemical nature from those of SE.
- 社団法人日本動物学会の論文
- 2002-11-25
著者
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Katagiri Nobuko
Medical Research Institute Tokyo Women's Medical University
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Katagiri Nobuko
Medical Research Institute Tokyo Women's Medical College
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Katagiri Yasuo
Section of Basic Science, Tokyo Women's Medical University School of Nursing
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Katagiri Y
Section Of Basic Science Tokyo Women's Medical University School Of Nursing
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Shimatani Yuichi
Department Of Physiology Tokyo Women's Medical University School Of Medicine
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Suzuki Tatsuo
Department Of Neuroplasticity Institute On Aging And Adaptation Shinshu University Graduate School O
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Suzuki Tatsuo
Department Of Pharmacology Hyogo College Of Medicine
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Suzuki Tatsuo
Department Of Biochemistry Nagoya City University Medical School
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Katagiri Yasuo
Section of Basic Science, Tokyo Women's Medical University School of Nursing
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Shimatani Yuichi
Department of Biomedical Engineering, Tokyo City University, Setagaya, Tokyo 158-8557, Japan
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