Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment
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概要
- 論文の詳細を見る
In our previous work we cloned on λ Charon4A phage vector a 6. 4-kb EcoRI fragment, which had the transforming activity of inducing gene amplification with a 16-kb repeating unit on the Bacillus subtilis chromosome by competence transformation ; those transformants were selected as tunicamycin resistant (Tm^r) transformants. We found that this DNA fragment can induce another type of gene amplification with a 8. 7-kb repeating unit by transformation when we use an amyE07 mutant as a recipient cell and select both Tm^r and amyE^+ transformants. In the latter (8.7-kb type) gene amplification, the copy number was increased up to 20 in contrast to about 10 copies in the former 16-kb type. We replaced a part of the 6.4 kb EcoRI fragment with a cat gene as a model of a foreign gene, and succeeded in the amplification of the cat gene on the chromosome by the latter type of transformation.
- 社団法人日本農芸化学会の論文
- 1993-01-23
著者
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Yamasaki M
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And
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YAMASAKI Makari
Department of Agricultural Chemistry, the University of Tokyo
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YODA Koji
Department of Biotechnology, University of Tokyo
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Yoda K
Univ. Tokyo Tokyo Jpn
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MORI Masaki
Department of Agricultural Chemistry, University of Tokyo
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TAMURA Gakuzo
Department of Agricultural Chemistry, University of Tokyo
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Yamane K
Univ. Tsukuba
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Mori M
Ajinomoto Co. Inc. Kawasaki Jpn
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Tamura Gakuzo
Depart Ment Qf Agricultural Chemistry The University Of Tokyo
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Koyama Nobuto
Department of Agricultural Chemistry, The University of Tokyo
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Shiozuka Kouichi
Department of Agricultural Chemistry, The University of Tokyo
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Yamaji Noboru
Department of Agricultural Chemistry, The University of Tokyo
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Yoda Koji
Department Of Agricultural Chemistry The University Of Tokyo
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Yamasaki Makari
Advanced Research Institute For The Science And Humanities Nihon University
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Yamasaki Makari
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura Gakuzo
Nuclear Receptor Institute Ksp
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Yamaji Noboru
Department Of Agricultural Chemistry The University Of Tokyo
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Koyama N
Hokkaido Univ. Sapporo Jpn
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MORI Masaaki
Life Science Research Center, Shiseido Co., Ltd.
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Mori M
Institute For Cosmic Ray Research University Ot Tokyo
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Shiozuka Kouichi
Department Of Agricultural Chemistry The University Of Tokyo
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Yoda K
Institute Of Biological Sciences University Of Tsukuba
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