Distribution of Prx-linked Hydroperoxide Reductase Activity among Microorganisms

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概要

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•   Peroxiredoxin (Prx) constitutes a large family of enzymes found in microorganisms, animals, and plants, but the detection of the activities of Prx-linked hydroperoxide reductases (peroxiredoxin reductases) in cell extracts, and the purification based on peroxide reductase activity, have only been done in bacteria and Trypanosomatidae. A peroxiredoxin reductase (NADH oxidase) from a bacterium, Amphibacillus, displayed only poor activities in the presence of purified Prx from Saccharomyces or Synechocystis, while it is highly active in the presence of bacterial Prx. These results suggested that an enzyme system different from that in bacteria might exist for the reduction of Prx in yeast and cyanobacteria.  Prx-linked hydroperoxide reductase activities were detected in cell extracts of Saccharomyces, Synechocystis, and Chlorella, and the enzyme activities of Saccharomyces and Chlorella were induced under vigorously aerated culture conditions and intensive light exposure conditions, respectively. Partial purification of Prx-linked peroxidase from the induced yeast cells indicated that the Prx-linked peroxidase system consists of two protein components, namely, thioredoxin and thioredoxin reductase. This finding is consistent with the previous report on its purification based on its protein protection activity against oxidation [Chae et al., J. Biol. Chem., 269, 27670–27678 (1994)]. In this study we have confirmed that Prx-linked peroxidase activity are widely distributed, not only in bacteria species and Trypanosomatidae, but also in yeast and photosynthetic microorganisms, and showed reconstitution of the activity from partially purified interspecies components.

• 社団法人 日本農芸化学会の論文
• 2004-01-23

著者

• TOKUE Chiyoko

  Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture

• YODA Koji

  Department of Biotechnology, University of Tokyo

• 新村 洋一

  Department Of Bioscience Tokyo University Of Agriculture

• NIIMURA Youichi

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Yoda Koji

  Department Of Biotechnology The University Of Tokyo

• Takeda Kouji

  Department of Bioscience, Tokyo University of Agriculture

• WATANABE Toshihiro

  Department of Food Science and Technology, Tokyo University of Agriculture

• KAWASAKI Shinji

  Department of Bioscience, Tokyo University of Agriculture

• Tokue Chiyoko

  Department Of Nutritional Science Faculty Of Applied Bio-science Junior College Of Tokyo University

• Tokue Chiyoko

  Department Of Nutritional Science Tokyo University Of Agriculture

• Yoda Koji

  Department Of Agricultural Chemistry The University Of Tokyo

• NISHIYMA Yoshitaka

  Department of Nutritional Science, Tokyo University of Agriculture

• NIMURA (MATSUNE)

  Department of Bioscience, Tokyo University of Agriculture

• MURA Kiyoshi

  Department of Nutritional Science, Tokyo University of Agriculture

• KATOH Tetzuya

  Department of Bioscience, Tokyo University of Agriculture

• Niimura Youichi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Watanabe Toshihiro

  Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture

• Mura Kiyoshi

  Department Of Nutritional Science Tokyo University Of Agriculture

• Katoh Tetzuya

  Department Of Bioscience Tokyo University Of Agriculture

• Kawasaki S

  Dep. Of Bioscience Tokyo Univ. Of Agriculture

• Yoshitaka Nishiyama

  Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Takeda Kouji

  Department Of Bioscience Tokyo University Of Agriculture

• Nimura (matsune)

  Department Of Bioscience Tokyo University Of Agriculture

• Kawasaki Shinji

  Department Of Bioscience Tokyo University Of Agriculture

• Watanabe Toshihiro

  Department Of Food Science And Technology Tokyo University Of Agriculture

• Watanabe Toshihiro

  Department Of Applied Mathematics University Of Gifu

• Nishiyama Yoshitaka

  Department Of Bioscience Tokyo University Of Agriculture

• Mura Kiyoshi

  Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture

• Nishiyama Yoshitaka

  Department Of Nutritional Science Faculty Of Applied Bio-science Tokyo University Of Agriculture

• KATOH TETZUYA

  Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo:(Present)Botanical Institute, Kyoto University

• NISHIYAMA Yoshitaka

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University

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