Separation of Cl-Containing Chlorophylls by Column Chromatography
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概要
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Using Toyopearl and cyclohexane : cyclohexanol solvent, four Cl-containing Chls were separated from ^<36>Cl-labeled cells of the blue-green, Plectonema boryanum. In normally grown cells, all four Cl-containing chlorophylls amounted to less than 1/2,000 of the total Chl and about 1/50 of P700, values much lower than previously reported contents of Chl RC I, and varied from alga to alga. The level of Cl-containing Chl was markedly enhanced when the cells were poisoned with methyl viologen. These results suggests that these Cl-containing Chls are not related to the reaction center of PS I.
著者
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Katoh Tetzuya
Department Of Bioscience Tokyo University Of Agriculture
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Yasuda Kyoko
Department of Botany, Faculty of Science, Kyoto University
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Yasuda Kyoko
Department Of Botany Faculty Of Science Kyoto University
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KATOH TETZUYA
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo:(Present)Botanical Institute, Kyoto University
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