Involvement of Sialic Acid in Transport of Serotype C1 Botulinum Toxins through Rat Intestinal Epithelial Cells
スポンサーリンク
概要
- 論文の詳細を見る
Clostridium botulinum produces a large toxin complex (L-TC) composed of neurotoxin (BoNT) and non-toxic proteins. In animal botulism, BoNT or L-TC is absorbed via the intestinal epithelium. To establish the cellular mechanisms of botulinum toxin absorption, we used cultured rat intestinal epithelial cells to test the binding and transport of serotype C1 BoNT and L-TC through the cell layers. BoNT and L-TC bound to and passed through the cell layers, with L-TC exhibiting larger binding and transport. Binding and transport of these toxins were inhibited by N-acetyl neuraminic acid or neuraminidase-treatment of the cells. These results suggest that binding of serotype C1 BoNT and L-TC to sialic acid on the cells promoted their transport through intestinal epithelial cell layers.
著者
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Niwa Koichi
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Ohyama Tohru
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Matsuo Tomohito
Department Of Medical Science And Technology School Of Medicine Kyushu University
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Ito Hiroaki
Department Of Anesthesiology And Medical Crisis Management Nagoya City University Graduate School Of
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Watanabe Toshihiro
Department Of Applied Mathematics University Of Gifu
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Ikeda Toshihiko
Yokohama Coll. Of Pharmacy
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INUI Ken
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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MIYATA Keita
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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HORIUCHI Ryohta
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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WATANABE Toshihiro
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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MIYATA Keita
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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NIWA Koichi
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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INUI Ken
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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ITO Hiroaki
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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