Purification and Characterization of Phospholipase D from Cabbage Leaves
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概要
- 論文の詳細を見る
Phospholipase D (PLD) was purified from cabbage leaves. The molecular weight of purified PLD was estimated as approximately 73 and 87 kDa by gel filtration using Superdex 200 HR column and, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. The transphosphatidylation capacity for phosphatidylcholine of this enzyme reached over 90% , and the enzymes hydrolysis efficiency for phosphatidylcholine was five-fold higher than that for phosphatidylglycerol. These findings indicated that the cabbage PLD efficiently transferred phosphatidylcholine to phosphatidylglycerol. On N-terminal amino acid sequence analysis of the band separated by SDSPAGE, two sequences with differing N-terminus were detected. This N-terminal difference may have been generated by processing during maturation of PLD.
- 社団法人 日本食品科学工学会の論文
- 2000-02-01
著者
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Nakazawa Yozo
Department Of Pediatrics Shinshu University School Of Medicine
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Nakazawa Yozo
Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agric
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WATANABE Toshihiro
Department of Food Science and Technology, Tokyo University of Agriculture
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SATO Hiroaki
Department of Internal Medicine, Wakayama Medical University Kihoku Hospital
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TAKANO Katsumi
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tsukuba
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SAGANE Yoshimasa
Department of Food Sciences and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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Watanabe Toshihiro
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Sato Hiroaki
Department Of Biosystem Management Division Of Environmental Conservation Graduate School Of Environ
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Takano K
Tokyo Univ. Agriculture Tokyo Jpn
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Takano Katsumi
Department Of Electrical Engineering Faculty Of Engineering Yamagata University:(present Address)hit
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Sagane Y
Tokyo Univ. Agriculture Hokkaido Jpn
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Sagane Yoshimasa
Department Of Food Sciences And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Takano Katsumi
Department Of Applied Biology And Chemistry Faculty Of Applied Bioscience Tokyo University Of Agricu
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Watanabe Toshihiro
Department Of Applied Mathematics University Of Gifu
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Sato H
National Inst. Of Advanced Industrial Sci. And Technol. (aist) Ibaraki Jpn
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Sato Hiroaki
Department Of Anesthesiology University Of Yamanashi Faculty Of Medicine
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Sato Hiroaki
Department Of Agricultural Chemistry Kyushu University
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SAGANE Yoshimasa
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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