Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase
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概要
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Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not JNK and ERK.
著者
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Niwa Koichi
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Ohyama Tohru
Department Of Food Science And Technology Faculty Of Bioindustry Tokyo University Of Agriculture
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Hayashi Shintaro
Department Of Earth Sciences Faculty Of Education And Human Studies Akita University
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Watanabe Toshihiro
Department Of Applied Mathematics University Of Gifu
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INUI Ken
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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Suzuki Tomonori
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
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SAGANE Yoshimasa
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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MIYATA Keita
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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OHYAMA Tohru
Department of Health and Nutrition, Faculty of Human Science, Hokkaido Bunkyo University, 5–196–1 Kogane-chuo, Eniwa, Hokkaido 061–1449, Japan
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MIYASHITA Shin-ichiro
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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NIWA Koichi
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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HAYASHI Shintaro
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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HAYASHI Shintaro
Department of Civil Engineering, Osaka Sangyo University, Japan
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INUI Ken
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099–2493, Japan
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- Botulinum Toxin Complex Increases Paracellular Permeability in Intestinal Epithelial Cells via Activation of p38 Mitogen-Activated Protein Kinase